Overview

  • Product nameAnti-NXF1 antibody
    See all NXF1 primary antibodies
  • Description
    Rabbit polyclonal to NXF1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 449-598 of Human NXF1

  • Positive control
    • 293T, A431, HeLaS3, HepG2, Molt-4, Raji and HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab96078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionInvolved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.
  • Tissue specificityExpressed ubiquitously.
  • Sequence similaritiesBelongs to the NXF family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 TAP-C domain.
  • DomainThe minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.
    The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.
    The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus.
    The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.
    The leucine-rich repeats are essential for the export of mRNA from the nucleus.
    The RNA-binding domain is a non-canonical RNP-type domain.
  • Cellular localizationNucleus > nucleoplasm. Nucleus speckle. Cytoplasm. Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp667O0311 antibody
    • DmNXF1 antibody
    • MEX67 antibody
    • MEX67, yeast, homolog of antibody
    • Mex67p antibody
    • mRNA export factor TAP antibody
    • Mvb1 antibody
    • Nuclear RNA export factor 1 antibody
    • Nuclear RNA export factor 1 homolog (S. cerevisiae) antibody
    • nxf 1 antibody
    • NXF1 antibody
    • NXF1_HUMAN antibody
    • Protein small bristles antibody
    • Sbr antibody
    • TAP antibody
    • Tip associating protein antibody
    • Tip-associated protein antibody
    • Tip-associating protein antibody
    see all

Anti-NXF1 antibody images

  • Anti-NXF1 antibody (ab96078) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 70 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96078 at 1/200 dilution.

References for Anti-NXF1 antibody (ab96078)

ab96078 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 40000 cells
Gel Running Conditions Reduced Denaturing (4-12% gradient gel)
Sample Zebrafish Tissue lysate - whole (10 embryos, animal cap)
Specification 10 embryos, animal cap
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Maximilian Krause

Verified customer

Submitted Jan 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"