Overview

  • Product name
    Anti-NXF3 antibody
  • Description
    Rabbit polyclonal to NXF3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 292-524 of Human NXF3 (Uniprot ID: Q9H4D5).

  • Positive control
    • NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; MCF7 cells; Human A549 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    May function as a tissue-specific nuclear mRNA export factor.
  • Tissue specificity
    Expressed at high level in testis and at low level in a small number of tissues.
  • Sequence similarities
    Belongs to the NXF family.
    Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain
    Lacks C-terminal domain that mediates direct interactions with nucleoporins.
    Contains a novel CRM1-dependent nuclear export signal that compensates in cis for the loss of the nuclear pore targeting domain.
    The RNA-binding domain is a non-canonical RNP-type domain.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Nuclear RNA export factor 3 antibody
    • NXF3 antibody
    • NXF3_HUMAN antibody
    • OTTHUMP00000023726 antibody
    • OTTHUMP00000023727 antibody
    • TAP like protein 3 antibody
    • TAP-like protein 3 antibody
    • TAPL 3 antibody
    • TAPL-3 antibody
    • TAPL3 antibody
    see all

Images

  • All lanes : Anti-NXF3 antibody (ab154916) at 1/10000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : IMR32 whole cell lysate
    Lane 3 : U-87 MG whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue labeling NXF3 with ab154916 at 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed MCF7 cells labeling NXF3 with ab154916 at 1/200 dilution (green). Lower image shows cells co-stained with Hoechst 33342.

References

ab154916 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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