The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay 1:312500.
FunctionMay function as a tissue-specific nuclear mRNA export factor.
Tissue specificityExpressed at high level in testis and at low level in a small number of tissues.
Sequence similaritiesBelongs to the NXF family. Contains 1 NTF2 domain. Contains 1 RRM (RNA recognition motif) domain.
DomainLacks C-terminal domain that mediates direct interactions with nucleoporins. Contains a novel CRM1-dependent nuclear export signal that compensates in cis for the loss of the nuclear pore targeting domain. The RNA-binding domain is a non-canonical RNP-type domain.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
Predicted band size : 60 kDa Western blot analysis of HeLa cystosolic (lane 1) and nuclear (lane 2) lysates (7µg/lane) labeling NXF3 with ab76430 at 1:300. A HRP-conjugated anti-rabbit (1:500) was used as the secondary antibody.
Western blot - NXF3 antibody (ab76430)
Anti-NXF3 antibody (ab76430) at 1 µg/ml + HepG2 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution