Overview

  • Product name
  • Description
    Rabbit polyclonal to NXF5
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide: TEKAPKVKTL NLSKNKLESA WELGKVKGLK LEELWLEGNP LCSTFSDQSA, corresponding to amino acids 181-230 of Human NXF5

  • Positive control
    • Jurkat cell lysate; Paraffin-embedded human kidney tissue, Paraffin-embedded human intestine tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab50954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    NXF5 is a member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. Five transcript variants that encode different isoforms have been found for the NXF5 gene.
  • Cellular localization
    Cytoplasmic and Nuclear. Mainly localized in the cytoplasm particularly in the cell body and neurites of hippocampal neurons. Nuclear localization is also observed. Not detected at nuclear rim.
  • Database links
  • Alternative names
    • Nuclear RNA export factor 5 antibody
    • TAP like protein 1 antibody
    • TAPL 1 antibody
    • TAPL1 antibody

Images

  • Anti-NXF5 antibody (ab50954) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • Tissue: paraffin-embedded human Kidney
    Cells with positive label: Epithelial cells of renal tubule (indicated with arrows)
    Antibody concentration 4 - 8 µg/ml
    Magnification: 400X
  • Tissue: paraffin-embedded human Intestine
    Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows)
    Antibody concentration: 4 - 8 µg/ml
    Magnification: 400X

References

ab50954 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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