Overview

  • Product name
    Anti-NXPH4 antibody
  • Description
    Rabbit polyclonal to NXPH4
  • Specificity
    ab74999 detects endogenous levels of total NXPH4 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    A synthesized peptide derived from an internal region of human NXPH4.

  • Positive control
    • IHC-P: human brain tissue. WB: extracts from COLO cells and HUVEC cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 33 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

Target

  • Relevance
    NXPH4 is a neurexophilin. Mammalian brains contain four different neurexophilin proteins. Neurexophilins form a family of related glycoproteins that are proteolytically processed after synthesis and bind to alpha neurexins. The structure and characteristics of neurexophilins indicate that they function as neuropeptides that may signal via alpha neurexins and possibly other receptors.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Neurexophilin 4 antibody
    • NPH4 antibody
    • NXPH 4 antibody

Images

  • All lanes : Anti-NXPH4 antibody (ab74999) at 1/500 dilution

    Lane 1 : Extracts from COLO cells
    Lane 2 : Extracts from HUVEC cells
    Lane 3 : Extracts from COLO cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • Immunohistochemical analysis of NXPH4 in paraffin embedded human brain tissue using ab74999 at a 1/50 dilution.
    Left image: untreated.
    Right image: treated with immunizing peptide.

References

ab74999 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab74999.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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