Overview

  • Product nameAnti-NXT1 antibody
    See all NXT1 primary antibodies
  • Description
    Rabbit polyclonal to NXT1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 41-90 (GTATLVWNGN AVSGQESLSE FFEMLPSSEF QISVVDCQPV HDEATPSQTT) of human NXT1 (NP_037380).

  • Positive control
    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab108233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-NXT1 antibody images

  • Anti-NXT1 antibody (ab108233) at 1 µg/ml + Fetal liver lysate at 10 µg

    Predicted band size : 16 kDa

References for Anti-NXT1 antibody (ab108233)

ab108233 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (293T cells)
Specification 293T cells
Fixative Formaldehyde
Permeabilization Yes - 0.2% triton x100 in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1%
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Submitted Nov 21 2011

Application Western blot
Sample Human Cell lysate - whole cell (293T cells)
Loading amount 20000 cells
Specification 293T cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 37°C
Username

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Verified customer

Submitted Nov 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"