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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-NXT2 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXT2 antibody (ab121797)
ab121797, at 1/450 dilution, staining NXT2 in paraffin-embedded Human pancreas tissue, by Immunohistochemistry.
Western blot - Anti-NXT2 antibody (ab121797)
All lanes : Anti-NXT2 antibody (ab121797) at 1/250 dilution Lane 1 : Negative control (Vector-only transfected HEK293T lysate).
Lane 2 : NXT2-transfected HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)) Developed using the ECL technique
References for Anti-NXT2 antibody (ab121797)
This product has been referenced in:
et al. MicroRNA-7-regulated TLR9 signaling-enhanced growth and metastatic potential of human lung cancer cells by altering the phosphoinositide-3-kinase, regulatory subunit 3/Akt pathway. Mol Biol Cell 24:42-55 (2013).
Read more (PubMed: 23135998) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"