Recombinant
RabMAb

Anti-NYREN18 antibody [EPR10716] (ab171077)

Overview

  • Product name
    Anti-NYREN18 antibody [EPR10716]
    See all NYREN18 primary antibodies
  • Description
    Rabbit monoclonal [EPR10716] to NYREN18
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NYREN18 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y5A7

  • Positive control
    • Raji, SHSY5Y, 293T and HeLa cell line lysates. HeLa cells. Permeabilized Raji cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10716
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 71 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.
    • Tissue specificity
      Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.
    • Sequence similarities
      Contains 3 UBA domains.
    • Cellular localization
      Nucleus. Predominantly nuclear.
    • Information by UniProt
    • Database links
    • Alternative names
      • BS4 antibody
      • NEDD8 ultimate buster 1 antibody
      • Negative regulator of ubiquitin like proteins 1 antibody
      • Negative regulator of ubiquitin-like proteins 1 antibody
      • NUB1 antibody
      • NUB1_HUMAN antibody
      • NUB1L antibody
      • NY REN 18 antibody
      • NYREN18 antibody
      • Renal carcinoma antigen NY-REN-18 antibody
      see all

    Images

    • All lanes : Anti-NYREN18 antibody [EPR10716] (ab171077) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : SHSY5Y cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 71 kDa

    • Immunofluorescent staining of HeLa cells labeling NUB1 using ab171077 at 1/100 dilution.

    • Flow cytometric analysis of permeabilized Raji cells labeling NUB1 using ab171077 at 1/10 dilution (red) or a rabbit IgG negative(green).

    References

    ab171077 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab171077.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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