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Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Our Abpromise guarantee covers the use of ab2739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23734074|
|ChIP/Chip||Use at an assay dependent concentration. PubMed: 20368426|
|WB||Use a concentration of 1 µg/ml.|
|IP||Use at an assay dependent concentration.|
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Jurkat cells were treated with either 50 uM PugNAc (ab144670) or 4 mM glucosamine + 50 uM PugNAc (ab144670) for three hours prior to harvest to stimulate O-linked glycosylation. The expected increase in glycosylation is observed in the treated lanes 2 & 4.
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