The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 23 kDa).
Use at 2-5 µg/mg of lysate.
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Antigen retrieval with Tris-EDTA pH9.0 is recommended for human tissues. Antigen retrieval with citrate buffer pH6.0 is recommended for mouse tissues.
RelevanceSingle-stranded DNA (ssDNA)-binding proteins, such as OBFC2B, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage.
OBFC2B plays an important role in DNA damage response. It influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. It associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins.
Cellular localizationNuclear. Localizes to nuclear foci following DNA damage.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling OBFC2B with ab85752 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - OBFC2B antibody (ab85752)
All lanes : Anti-OBFC2B antibody (ab85752) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Detection of Human OBFC2B by Western Blot of Immunprecipitate. All lanes: ab85752 at 1µg/ml detecting by WB OBFC2B in HeLa whole cell lysates, that were immunoprecipitated with ab85752 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 1: ab85752 Lane 2: Control IgG Detection: Chemiluminescence with exposure time of 5 seconds.
References for Anti-OBFC2B antibody (ab85752)
This product has been referenced in:
Chen ZW et al. FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic Acids Res42:11560-9 (2014).
Read more (PubMed: 25249620) »