• Product nameAnti-OBFC2B antibody
    See all OBFC2B primary antibodies
  • Description
    Rabbit polyclonal to OBFC2B
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 161 and 211 of human OBFC2B (NP_076973.1)

  • Positive control
    • HeLa and 293T whole cell lysates.


Associated products


Our Abpromise guarantee covers the use of ab85752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 23 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Antigen retrieval with Tris-EDTA pH9.0 is recommended for human tissues. Antigen retrieval with citrate buffer pH6.0 is recommended for mouse tissues.


  • RelevanceSingle-stranded DNA (ssDNA)-binding proteins, such as OBFC2B, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage. OBFC2B plays an important role in DNA damage response. It influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. It associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins.
  • Cellular localizationNuclear. Localizes to nuclear foci following DNA damage.
  • Database links
  • Alternative names
    • 2610036N15Rik antibody
    • hSSB1 antibody
    • LP3587 antibody
    • MGC125161 antibody
    • MGC2731 antibody
    • MGC30353 antibody
    • Nabp2 antibody
    • Nucleic acid binding protein 2 antibody
    • Oligonucleotide/oligosaccharide binding fold containing 2B antibody
    • Oligonucleotide/oligosaccharide binding fold containing protein 2B antibody
    • RGD1308158 antibody
    • Sensor of single strand DNA complex subunit B1 antibody
    • Sensor of ssDNA subunit B1 antibody
    • Single stranded DNA binding protein 1 antibody
    • SOSS complex subunit B1 antibody
    • SSB1 antibody
    see all

Anti-OBFC2B antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling OBFC2B with ab85752 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-OBFC2B antibody (ab85752) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size : 23 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human OBFC2B by Western Blot of Immunprecipitate.
    All lanes: ab85752 at 1µg/ml detecting by WB OBFC2B in HeLa whole cell lysates, that were immunoprecipitated with ab85752 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 1: ab85752 Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 5 seconds.

References for Anti-OBFC2B antibody (ab85752)

This product has been referenced in:
  • Chen ZW  et al. FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic Acids Res 42:11560-9 (2014). Read more (PubMed: 25249620) »

See 1 Publication for this product

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