The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionInvolved in myofibrillogenesis. Seems to be involved in assembly of myosin into sarcomeric A bands in striated muscle. Isoform 3 together with ANK1 isoform Mu17/Ank1.5 may provide a molecular link between the sarcoplasmic reticulum and myofibrils.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 DH (DBL-homology) domain. Contains 3 fibronectin type-III domains. Contains 55 Ig-like (immunoglobulin-like) domains. Contains 1 IQ domain. Contains 2 PH domains. Contains 2 protein kinase domains. Contains 1 SH3 domain.
Cellular localizationCytoplasm > myofibril > sarcomere > M line. Cytoplasm > myofibril > sarcomere > Z line. In differentiating skeletal muscle cells, isoform 3 primarily localizes to the sarcomeric M-line and less frequently to the Z-disk. Isoform 3 colocalizes with ANK1 isoform Mu17/ank1.5 at the M-line in differentiated skeletal muscle cells.