Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
Our Abpromise guarantee covers the use of ab15112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EMSA||Use at an assay dependent concentration. PubMed: 20143233|
|WB||Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 89 kDa).|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab15112 staining Oct-1 in human tonsil by Immunohistochemistry (FFPE-sections).
This image is courtesy of an anonymous AbreviewBlocking Step: 5% Milk for 1 hour at 22°C
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"