Overview

  • Product nameAnti-Oct-1 antibody [POU5I097]
    See all Oct-1 primary antibodies
  • Description
    Mouse monoclonal [POU5I097] to Oct-1
  • Tested applicationsSuitable for: Flow Cyt, Dot blot, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Oct-1.
    Database link: P14859

  • Positive control
    • HeLa cells, HEK293 whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 99% PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesFiltered through a 0.22 µm membrane.
  • ClonalityMonoclonal
  • Clone numberPOU5I097
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Dot blot Use at an assay dependent concentration.
WB 1/50. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
ICC/IF 1/10.

Target

  • FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
  • Tissue specificityUbiquitous. Isoform 2 is lymphocyte-specific.
  • Sequence similaritiesBelongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ42836 antibody
    • NF A1 antibody
    • NF-A1 antibody
    • Oct 1 antibody
    • Oct 1B antibody
    • Oct-1 antibody
    • OCT1 antibody
    • Octamer binding protein 1 antibody
    • Octamer binding transcription factor 1 antibody
    • Octamer-binding protein 1 antibody
    • Octamer-binding transcription factor 1 antibody
    • OTF 1 antibody
    • OTF-1 antibody
    • OTF1 antibody
    • OTTHUMP00000032348 antibody
    • OTTHUMP00000032350 antibody
    • OTTHUMP00000032351 antibody
    • PO21 antibody
    • PO2F1 antibody
    • PO2F1_HUMAN antibody
    • POU class 2 homeobox 1 antibody
    • POU domain class 2 transcription factor 1 antibody
    • POU domain, class 2, transcription factor 1 antibody
    • POU2F1 antibody
    see all

Anti-Oct-1 antibody [POU5I097] images

  • Ab51363 at 1:10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1:200 dilution.

  • Anti-Oct-1 antibody [POU5I097] (ab51363) at 1/50 dilution + HEK293 whole cell lysate at 50 µg

    Secondary
    Anti mouse IgG antibody at 1/2500 dilution

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
  • Overlay histogram showing Jurkat cells stained with ab51363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51363, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Oct-1 antibody [POU5I097] (ab51363)

This product has been referenced in:
  • Thum T & Borlak J Lox-1 receptor blockade abrogates OXLDL induced oxidative DNA damage and prevents activation of the transcriptional repressor OCT-1 in human coronary arterial endothelium. J Biol Chem : (2008). ICC/IF ; Human . Read more (PubMed: 18390905) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

In brief, the protocol was: Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with ab51363.

...

Read More

Thank you for your inquiry. I am sorry to hear that you are having trouble with this antibody.

We have not tested, nor do we guarantee, this antibody to work in mouse. We have only tested this antibody with human samples. It may be that this ...

Read More

Expiration date: 30-November-2012

I am very pleased to hear you would like to accept our offer and test ab15112, ab51363 and ab66132in Flow Cytometry. These codes will give you:3 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the...

Read More

Thank you for contacting us.

Could you let me know the sample type, species and application e.g. WB, IHC-P customer is interested in?

Please note the abreview discount code is valid only for untested species and applications. When se...

Read More

Thank you for your interest.

We have some antibodies against Oct1 but currently none of them have been tested in Flow cytometry. These products have been characterized in IHC and WB.

http://www.abcam.com/ab15112: Anti-Oct1 antibody ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"