The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.
Use at 2 µg/mg of lysate.
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionTranscription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
Tissue specificityIsoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
Sequence similaritiesBelongs to the POU transcription factor family. Class-2 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.