Overview

  • Product name
  • Description
    Rabbit polyclonal to Oct-2
  • Specificity
    This antibody recognises Oct-2. It may also detect the isoform 2, according to sequence analysis.
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Oct-2 (N terminal).
    Database link: P09086

  • Positive control
    • WB: Raji cell lysate. IHC-P: Human lymph node tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Affinity Purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/20. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
    • Tissue specificity
      Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
    • Sequence similarities
      Belongs to the POU transcription factor family. Class-2 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • class 2 antibody
      • Lymphoid restricted immunoglobulin octamer binding protein antibody
      • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
      • NF A2 antibody
      • Oct 2 antibody
      • Oct-2 antibody
      • Octamer binding transcription factor 2 antibody
      • Octamer-binding protein 2 antibody
      • Octamer-binding transcription factor 2 antibody
      • OTF-2 antibody
      • OTF2 antibody
      • PO2F2_HUMAN antibody
      • POU domain antibody
      • POU domain class 2 transcription factor 2 antibody
      • POU2F2 antibody
      • transcription factor 2 antibody
      see all

    Images

    • Ab32156, at a 1:250 dilution, staining Oct-2 in paraffin embedded human lymph node tissue by Immunohistochemistry.

      Ab32156, at a 1/250 dilution, staining Oct2 in paraffin embedded human lymph node tissue by Immunohistochemistry.
    • Anti-Oct-2 antibody (ab32156) at 1/1000 dilution + Raji cell lysate

      Predicted band size : 51 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)

    References

    ab32156 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab32156.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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