Recombinant Anti-Oct-2 antibody [EPR12482] (ab179808)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12482] to Oct-2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Oct-2 antibody [EPR12482]
See all Oct-2 primary antibodies -
Description
Rabbit monoclonal [EPR12482] to Oct-2 -
Host species
Rabbit -
Specificity
The Oct-2 targeted by ab179808 is POU domain, class 2, transcription factor 2 (SwissProt ID: P09086).
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Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details
Unsuitable for: ChIP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Oct-2. The exact sequence is proprietary.
Database link: P09086 -
Positive control
- WB: Raji whole cell lysate. Rat and mouse brain tissue lysates; IP: Raji whole cell lysate; ICC/IF: Ramos; Flow-cyt: Raji cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12482 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100 - 1/500. |
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WB |
1/5000. Predicted molecular weight: 51 kDa.
For unpurified use at 1/1000 - 1/5000. |
|
ICC/IF |
1/200.
For unpurified use at 1/100 - 1/250. |
|
IP |
1/30.
For unpurified use at 1/10 - 1/100. |
Notes |
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Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100 - 1/500. |
WB
1/5000. Predicted molecular weight: 51 kDa. For unpurified use at 1/1000 - 1/5000. |
ICC/IF
1/200. For unpurified use at 1/100 - 1/250. |
IP
1/30. For unpurified use at 1/10 - 1/100. |
Target
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Function
Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. -
Tissue specificity
Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147. -
Sequence similarities
Belongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5452 Human
- Entrez Gene: 18987 Mouse
- Entrez Gene: 117058 Rat
- Omim: 164176 Human
- SwissProt: P09086 Human
- SwissProt: Q00196 Mouse
- Unigene: 147381 Human
- Unigene: 76683 Mouse
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Alternative names
- class 2 antibody
- Lymphoid restricted immunoglobulin octamer binding protein antibody
- Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
see all
Images
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All lanes : Anti-Oct-2 antibody [EPR12482] (ab179808) at 1/5000 dilution (Purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST
The observed bands are consistent with what are described in PMID: 26993806.
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Purified ab179808 at 1/30 dilution (2ug) immunoprecipitating Oct-2 in Raji whole cell lysate.
Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (10µg)
Lane 2 (+): ab179808 + Raji whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab179808 in Raji whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 58 kDa -
Immunocytochemistry/ Immunofluorescence analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling Oct-2 with purified ab179808 at 1/200 (2.7 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling Oct-2 with purified ab179808 at 1/50 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab179808 has been referenced in 11 publications.
- Wang Q et al. Mechanisms Underlying the Differences in the Pharmacokinetics of Six Active Constituents of Huangqi Liuyi Decoction between Normal and Diabetic Nephropathy Mouse Models. Evid Based Complement Alternat Med 2022:2481654 (2022). PubMed: 36285162
- Cong X et al. Silencing GnT-V reduces oxaliplatin chemosensitivity in human colorectal cancer cells through N-glycan alteration of organic cation transporter member 2. Exp Ther Med 21:128 (2021). PubMed: 33376510
- Luo R et al. POU2F2 promotes the proliferation and motility of lung cancer cells by activating AGO1. BMC Pulm Med 21:117 (2021). PubMed: 33832481
- Jiang J et al. Bacterial infection reinforces host metabolic flux from arginine to spermine for NLRP3 inflammasome evasion. Cell Rep 34:108832 (2021). PubMed: 33691113
- Tamassia N et al. Induction of OCT2 contributes to regulate the gene expression program in human neutrophils activated via TLR8. Cell Rep 35:109143 (2021). PubMed: 34010659