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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> OCT
Overview
Product name
Anti-Oct4 antibody
See all Oct4 products (19) ...
Description
Rabbit polyclonal to Oct4
Tested applications
IHC-Fr, WB, IP, ICC/IF, Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human, Pig
Immunogen
Recombinant fragment, corresponding to amino acids 1-14 of Human Oct4(Peptide available as ab52110.)
Positive control
F9 whole cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> OCT
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
ICC/IF
Flow Cyt
Application notes
ICC/IF: Use at an assay dependent dilution (PMID 19736565).
IHC-P: 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at a concentration of 20 µg/ml.
IP: Use at a concentration of 20 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa. Can be blocked with Oct4 peptide (ab52110).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Tissue specificity
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Sequence similarities
Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Developmental stage
Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
Domain
The POU-specific domain mediates interaction with PKM2.
Post-translational
modifications
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
Cellular localization
Nucleus. Expressed in a diffuse and slightly punctuate pattern.
Target information above from: UniProt accessionQ01860
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC22487 antibody
- Oct 3 antibody
- Oct 4 antibody
see all
Database links
- Entrez Gene: 282316 Cow
- Entrez Gene: 5460 Human
- Entrez Gene: 18999 Mouse
- Entrez Gene: 294562 Rat
- Omim: 164177 Human
- SwissProt: O97552 Cow
- SwissProt: Q01860 Human
- SwissProt: P20263 Mouse
see all
Anti-Oct4 antibody images:
Western blot - Oct4 antibody (ab18976)
Anti-Oct4 antibody (ab18976) at 1/500 dilution + mouse embryonic stem cell whole cell lysate at 100 µg/ml
Secondary
HRP conjugated goat anti-rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 45 kDa
Observed band size : 45 kDa
Additional bands at : 25 kDa (possible non-specific binding),60 kDa (possible non-specific binding).
Exposure time : 1 minute
The blot was blocked with 5% milk for 1 hour at RT and inbubated with the primary antibody for 16 hours at 4°C.
This image is courtesy of an anonymous Abreview
Western blot - Oct4 antibody (ab18976)
Anti-Oct4 antibody (ab18976) at 0.5 µg/ml + Lysates prepared from rat kidney tissue.
Predicted band size : 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oct4 antibody (ab18976)
ab18976 at 1/100 dilution staining Oct4 in mouse brain tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 5% BSA for 1 hour at 25°C and then incubated with ab18976 for 12 hours at 4°C. A Cy3 conjugated donkey polyclonal to rabbit Ig, diluted 1/500, was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Protocols
References for Anti-Oct4 antibody (ab18976)
This product has been referenced in:
- Tavernier Get al. Activation of pluripotency-associated genes in mouse embryonic fibroblasts by non-viral transfection with in vitro-derived mRNAs encoding Oct4, Sox2, Klf4 and cMyc. Biomaterials : (2011). ICC/IF, Flow Cyt; Mouse.Read more (PubMed: 21993235) »
- Wissing Set al. Endogenous APOBEC3B Restricts LINE-1 Retrotransposition in Transformed Cells and Human Embryonic Stem Cells. J Biol Chem 286:36427-37 (2011).Read more (PubMed: 21878639) »
See all 11 publications for this product
Publishing research using ab18976? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"