Overview

  • Product nameAnti-Oct4 antibody
    See all Oct4 primary antibodies
  • Description
    Rabbit polyclonal to Oct4
  • SpecificityThe antibody reacts with 45 kDa Oct4 (also designated Oct3A) and in a less extent 33 kDa Oct3B from human, mouse, and rat samples.
  • Tested applicationsSuitable for: IHC-Fr, WB, IP, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig, Macaque Monkey
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
    (Peptide available as ab52110)

  • Positive control
    • Rat kidney tissue lysate can be used as a positive control.

Properties

Applications

Our Abpromise guarantee covers the use of ab18976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa.Can be blocked with Oct4 peptide (ab52110).
IP Use a concentration of 20 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 19736565
Flow Cyt Use at an assay dependent concentration. PubMed: 17719011

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Tissue specificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Developmental stageHighly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • DomainThe POU-specific domain mediates interaction with PKM2.
  • Post-translational
    modifications
    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • Cellular localizationNucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Information by UniProt
  • Database links
  • Alternative names
    • Octamer binding transcription factor 4 antibody
    • MGC22487 antibody
    • Oct 3 antibody
    • Oct 4 antibody
    • Oct-3 antibody
    • Oct-4 antibody
    • OCT3 antibody
    • Oct4 antibody
    • Octamer binding protein 3 antibody
    • Octamer binding protein 4 antibody
    • Octamer binding transcription factor 3 antibody
    • Octamer-binding protein 3 antibody
    • Octamer-binding protein 4 antibody
    • Octamer-binding transcription factor 3 antibody
    • OTF 3 antibody
    • OTF 4 antibody
    • OTF-3 antibody
    • OTF3 antibody
    • OTF4 antibody
    • PO5F1_HUMAN antibody
    • POU class 5 homeobox 1 antibody
    • POU domain class 5 transcription factor 1 antibody
    • POU domain transcription factor OCT4 antibody
    • POU domain, class 5, transcription factor 1 antibody
    • POU-type homeodomain-containing DNA-binding protein antibody
    • POU5F1 antibody
    see all

Anti-Oct4 antibody images

  • Immunohistochemistry analysis of paraffin-embedded human breast tissues (5 µm thick) labeling Oct4 with ab18976 at 1/100 dilution.

    Tissues were rehydrated in deionized water and incubated in PBS prior to per meabilization in 0.1% PBS-TritonX 100 and overnight incubation with ab18976 in a humid chamber.Washing in PBS and secondary antibody (Alexa Fluor®) incubation for 2h was followed by a final wash and mounting.

    Blue: DAPI stain for nuclei.

    Minimal and mostly cytoplasmic expression was seen in the normal resting breast (left panel). During normal lactation (right panel), OCT4 was upregulated and expressed in both the nucleus and cytoplasm.

  • Anti-Oct4 antibody (ab18976) at 1/500 dilution + mouse embryonic stem cell whole cell lysate at 100 µg/ml

    Secondary
    HRP conjugated goat anti-rabbit
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 25 kDa (possible non-specific binding),60 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    The blot was blocked with 5% milk for 1 hour at RT and inbubated with the primary antibody for 16 hours at 4°C.

    See Abreview

  • Immunohistochemical analysis of paraffin embedded Human breast tissues (5 µm thick) labeling Oct4 with ab18976 at 1/100 to 1/200 dilution. Heat induced epitope retrieval was performed at 100°C for 30 min. Slides were counterstained with hematoxylin.

    Minimal and mostly cytoplasmic expression was seen in the normal resting breast (left panel). During normal lactation (right panel), OCT4 was upregulated and expressed in both the nucleus and cytoplasm.

  • ab18976 at 1/100 dilution staining Oct-4 in mouse brain tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 5% BSA for 1 hour at 25°C and then incubated with ab18976 for 12 hours at 4°C. A Cy3 conjugated donkey polyclonal to rabbit Ig, diluted 1/500, was used as the secondary antibody.

    See Abreview

  • Anti-Oct4 antibody (ab18976) at 0.5 µg/ml + Lysates prepared from rat kidney tissue.

    Predicted band size : 45 kDa

    The antibody reacts with 45 kDa Oct4 (also designated Oct-3A) and in a less extent 33 kDa Oct-3B from human, mouse, and rat samples.

References for Anti-Oct4 antibody (ab18976)

This product has been referenced in:
  • Liu H  et al. Spheres from cervical cancer cells display stemness and cancer drug resistance. Oncol Lett 12:2184-2188 (2016). Read more (PubMed: 27602161) »
  • Feng W  et al. Isolation and Identification of Prepubertal Buffalo (Bubalus bubalis) Spermatogonial Stem Cells. Asian-Australas J Anim Sci 29:1407-15 (2016). IF . Read more (PubMed: 26954139) »

See all 34 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Neural stem cells)
Permeabilization No
Specification Neural stem cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Ms. kyungjoo seong

Verified customer

Submitted Oct 31 2016

Application Flow Cytometry
Sample Human Cell (H9 hESCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Fibroblasts (white)
Specification H9 hESCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 24 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Fetal Brain)
Antigen retrieval step Other
Permeabilization No
Specification Fetal Brain
Blocking step Dako protein block as blocking agent for 10 minute(s) · Concentration: .25% · Temperature: 20°C
Fixative Formaldehyde
Username

Ms. Erica Sloma

Verified customer

Submitted Oct 09 2014

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody. The details your customer has kindly provided will enab...

Read More

Thank you for your enquiry and your interest.

I can confirm that two isoforms of Oct 4 have been identified, and the exact amino acid sequences (aa) can be found at this website: http://www.uniprot.org/uniprot/Q01860

- Isoform A: Q0...

Read More

Muchas gracias por tu respuesta.

Es importante que todos los anticuerpos que escojas para llevar a cabo IHC-P en tejido humano estén validados (y por tanto garantizados por Abcam) para funcionar en esta especie y aplicación. Est...

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Thank you for contacting us.

Please advice customer to consult publication whole selecting the antibodies.

HEK293T cells do not express c-Myc and Oct4. They express SOX2.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC128941...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cat Tissue sections (epithelial cancer, small intestine, testis)
Specification epithelial cancer, small intestine, testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step universal blocking reagent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 30 2011

Thank you very much for your email. I apologize for this confusion. Unfortunately I do not know where it comes from. I can however confirm that the immunogen used for this antibody is from amino acid 1 to 14. The sequence is AGHLASDFAFSPC. ...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (canine mast cell tumor)
Specification canine mast cell tumor
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, high pH, and protease
Permeabilization No
Blocking step universal reagent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 24 2011

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"