The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Tissue specificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.
Developmental stageHighly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
DomainThe POU-specific domain mediates interaction with PKM2.
Post-translational modificationsSumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
Cellular localizationNucleus. Expressed in a diffuse and slightly punctuate pattern.
POU domain class 5 transcription factor 1 antibody
POU domain transcription factor OCT4 antibody
POU domain, class 5, transcription factor 1 antibody
POU-type homeodomain-containing DNA-binding protein antibody
Anti-Oct4 antibody images
Western blot - Anti-Oct4 antibody (ab49091)
Anti-Oct4 antibody (ab49091) at 1.25 µg/ml (in 5% skimmed milk/PBS) + Jurkat cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 39 kDa Observed band size : 39 kDa Additional bands at : 35 kDa (possible isoform).
References for Anti-Oct4 antibody (ab49091)
This product has been referenced in:
Li Y et al. BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state. Development140:3965-76 (2013).
Read more (PubMed: 24004950) »
Kaeffer B et al. Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells. PLoS One6:e25562 (2011).
Read more (PubMed: 22028779) »