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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> OCT
Overview
Product name
Anti-Oct4 antibody - Embryonic Stem Cell Marker
See all Oct4 products (19) ...
Description
Goat polyclonal to Oct4 - Embryonic Stem Cell Marker
Tested applications
Flow Cyt, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from within residues 300 to the C-terminus of Human Oct4.
(Peptide available as ab20650.)
Positive control
Human or Mouse Embryonic Stem Cells
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> OCT
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent concentration.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
Target
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Tissue specificity
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Sequence similarities
Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Developmental stage
Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
Domain
The POU-specific domain mediates interaction with PKM2.
Post-translational
modifications
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
Cellular localization
Nucleus. Expressed in a diffuse and slightly punctuate pattern.
Target information above from: UniProt accessionQ01860
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC22487 antibody
- Oct 3 antibody
- Oct 4 antibody
see all
Database links
- Entrez Gene: 5460 Human
- Entrez Gene: 18999 Mouse
- Entrez Gene: 294562 Rat
- Omim: 164177 Human
- SwissProt: Q01860 Human
- SwissProt: P20263 Mouse
- Unigene: 249184 Human
- Unigene: 632482 Human
see all
Anti-Oct4 antibody - Embryonic Stem Cell Marker images:
Western blot - Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
All lanes : Anti-Oct4 antibody - Embryonic Stem Cell Marker (ab27985) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit polyclonal to Goat IgG (Alexa Fluor® 680) (ab28445) at 1/10000 dilution
Predicted band size : 39 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
The antibody recognised a band corresponding to Oct4 in mouse and human embryonic stem cell lysates.
Immunocytochemistry/ Immunofluorescence - Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
ab27985 recognizes Oct4 in human embryonic stem cells. The upper panel shows DAPI staining while the lower panel shows ab27985 staining.
This image is courtesy of an Abreview submitted by Dr Maria Keramari
Immunocytochemistry/ Immunofluorescence - Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
ab27985 recognizes Oct4 in mouse embryonic stem cells. DAPI staining is shown in the top panel while ab27985 staining is shown in the lower panel.
This image is courtesy of an Abreview submitted by Dr Maria Keramari
Flow Cytometry - Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
ab27985 staining Oct4 in human embryonic carcinoma cells by Flow Cytometery. Cells were fixed in paraformaldehyde and permeabilized in saponin buffer. The primary antibody was diluted 1/500 (PBS + 10% BSA) and incubated with sample for 1 hour at 4°C. A FITC conjugated Donkey polyclonal to goat IgG, diluted 1/200 was used as secondary. The red histogram is secondary only control whilst green and blue repersents Oct4 expression in untreated and partially differentiated with 10nM Trans-Retinoic acid for 14 days.
This image is courtesy of an Abreview submitted by Mr Mark Jones
References for Anti-Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
This product has been referenced in:
- Tolar Jet al. Hematopoietic differentiation of induced pluripotent stem cells from patients with mucopolysaccharidosis type I (Hurler syndrome). Blood 117:839-47 (2011). ICC/IF; Human.Read more (PubMed: 21037085) »
- Moreno-Manzano Vet al. FM19G11, a new hypoxia-inducible factor (HIF) modulator, affects stem cell differentiation status. J Biol Chem 285:1333-42 (2010). WB; Human.Read more (PubMed: 19897487) »
See all 9 publications for this product
Publishing research using ab27985? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"