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Western blot - Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
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Immunocytochemistry/ Immunofluorescence - Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
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Immunocytochemistry/ Immunofluorescence - Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
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Product Name
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Oct4 antibody - Embryonic Stem Cell Marker
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See all Oct4 antibodies (15)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Oct4 - Embryonic Stem Cell Marker
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Immunogen
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Synthetic peptide derived from within residues 300 to the C-terminus of Human Oct4.
(Peptide available as ab20650.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human or Mouse Embryonic Stem Cells
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> OCT
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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Relevance
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Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation. Oct4 is renowned as a transcription factor expressed by undifferentiated embryonic stem cells and embryonic germ cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Oct4 antibody - Embryonic Stem Cell Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Oct4 antibody - Embryonic Stem Cell Marker images, references, products related to ab27985 and other tools.
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Oct4 antibody - Embryonic Stem Cell Marker images:
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 Western blot - Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
All lanes : Oct4 antibody - Embryonic Stem Cell Marker (ab27985) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit polyclonal to Goat IgG (Alexa Fluor® 680) (ab28445) at 1/10000 dilution
Predicted band size : 39 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
The antibody recognised a band corresponding to Oct4 in mouse and human embryonic stem cell lysates.
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Immunocytochemistry/ Immunofluorescence - Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
ab27985 recognizes Oct4 in human embryonic stem cells. The upper panel shows DAPI staining while the lower panel shows ab27985 staining. This image is courtesy of an Abreview submitted by Dr Maria Keramari
See Abreview
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Immunocytochemistry/ Immunofluorescence - Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
ab27985 recognizes Oct4 in mouse embryonic stem cells. DAPI staining is shown in the top panel while ab27985 staining is shown in the lower panel. This image is courtesy of an Abreview submitted by Dr Maria Keramari
See Abreview
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Flow Cytometry - Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
ab27985 staining Oct4 in human embryonic carcinoma cells by Flow Cytometery. Cells were fixed in paraformaldehyde and permeabilized in saponin buffer. The primary antibody was diluted 1/500 (PBS + 10% BSA) and incubated with sample for 1 hour at 4°C. A FITC conjugated Donkey polyclonal to goat IgG, diluted 1/200 was used as secondary. The red histogram is secondary only control whilst green and blue repersents Oct4 expression in untreated and partially differentiated with 10nM Trans-Retinoic acid for 14 days. This image is courtesy of an Abreview submitted by Mr Mark Jones
See Abreview
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References for Oct4 antibody - Embryonic Stem Cell Marker (ab27985)
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This product has been used in: (two most recent references)
Moreno-Manzano V et al. FM19G11, a new hypoxia-inducible factor (HIF) modulator, affects stem cell differentiation status. J Biol Chem 285:1333-42 (2010). WB; Human. PubMed: 19897487
Moreno-Manzano V et al. Activated spinal cord ependymal stem cells rescue neurological function. Stem Cells 27:733-43 (2009). WB; Rat. PubMed: 19259940
See all 6 publication references for this product.
If you publish research using ab27985 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Oct4
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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