Recombinant
RabMAb

Anti-Oct4 antibody [EPR2054] (ab109183)

Overview

  • Product name
    Anti-Oct4 antibody [EPR2054]
    See all Oct4 primary antibodies
  • Description
    Rabbit monoclonal [EPR2054] to Oct4
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Oct4 aa 250-350.

  • Positive control
    • Seminoma, NCCIT and HeLa cell lysates; Human ovarian dysgerminoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Oct4 antibody (Alexa Fluor® 488) [EPR2054] (ab194074)
    Anti-Oct4 antibody (HRP) [EPR2054] (ab194076)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
    • Tissue specificity
      Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
    • Sequence similarities
      Belongs to the POU transcription factor family. Class-5 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Developmental stage
      Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
    • Domain
      The POU-specific domain mediates interaction with PKM2.
    • Post-translational
      modifications
      Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
      Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
    • Cellular localization
      Nucleus. Expressed in a diffuse and slightly punctuate pattern.
    • Information by UniProt
    • Database links
    • Alternative names
      • Octamer binding transcription factor 4 antibody
      • MGC22487 antibody
      • Oct 3 antibody
      • Oct 4 antibody
      • Oct-3 antibody
      • Oct-4 antibody
      • OCT3 antibody
      • Oct4 antibody
      • Octamer binding protein 3 antibody
      • Octamer binding protein 4 antibody
      • Octamer binding transcription factor 3 antibody
      • Octamer-binding protein 3 antibody
      • Octamer-binding protein 4 antibody
      • Octamer-binding transcription factor 3 antibody
      • OTF 3 antibody
      • OTF 4 antibody
      • OTF-3 antibody
      • OTF3 antibody
      • OTF4 antibody
      • PO5F1_HUMAN antibody
      • POU class 5 homeobox 1 antibody
      • POU domain class 5 transcription factor 1 antibody
      • POU domain transcription factor OCT4 antibody
      • POU domain, class 5, transcription factor 1 antibody
      • POU-type homeodomain-containing DNA-binding protein antibody
      • POU5F1 antibody
      see all

    Anti-Oct4 antibody [EPR2054] images

    • All lanes : Anti-Oct4 antibody [EPR2054] (ab109183) at 1/1000 dilution

      Lane 1 : Seminoma lysate
      Lane 2 : NCCIT cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 39 kDa
    • ab109183, at 1:500 dilution, staining Oct4 in paraffin-embedded Human ovarian dysgerminoma by Immunohistochemistry.

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling Oct4 with ab109183 at 1/60 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • ab109183 at 1/50 immunoprecipitating Oct4 in NCCIT (human pluripotent embryonal carcinoma) whole cell lysate observed at 39 KDa (lanes 1 and 2).

      Lane 1 (input): NCCIT whole cell lysate, 10μg

      Lane 2 (+): ab109183 + NCCIT whole cell lysate

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109183 in NCCIT whole cell lysate

      For western blotting, ab109183 at 1/1000 dilution and ab131366 VeriBlot for IP (HRP) was used as the secondary antibody at 1/10000.

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

       

       

    • Flow cytometry analysis of NCCIT (human pluripotent embryonal carcinoma cells labelling Oct4 (pink) with ab109183 at dilution of 1/100. The secondary antibody used was goat anti rabbit IgG (FITC) at dilution of 1/150. Cells were fixed with 2% paraformaldehyde. Isotype control antibody used was rabbit monoclonal IgG (green).

    • ab109183 showing negative staining in Colonic adenocarcinoma tissue.

    • ab109183 showing negative staining in Skeletal muscle tissue.

    • ab109183 showing negative staining in Normal liver tissue.

    • ab109183 showing positive staining in Seminoma tissue.

    • ab109183 showing negative staining in Normal brain tissue.

    • ab109183 showing positive staining in Fetal brain tissue.

    • ab109183 showing negative staining in Hepatocellular carcinoma tissue.

    • ab109183 showing positive staining in Embryonal carcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Oct4 antibody [EPR2054] (ab109183)

    This product has been referenced in:
    • Ram RS  et al. Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System. Front Surg 4:30 (2017). IHC ; Human . Read more (PubMed: 28634582) »
    • Ram R  et al. The Identification of Three Cancer Stem Cell Subpopulations within Moderately Differentiated Lip Squamous Cell Carcinoma. Front Surg 4:12 (2017). Read more (PubMed: 28321397) »

    See all 9 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Human Purified protein (brain cancer)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    brain cancer
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 24 2015

    Thank you for your enquiry and your interest.

    I can confirm that two isoforms of Oct 4 have been identified, and the exact amino acid sequences (aa) can be found at this website: http://www.uniprot.org/uniprot/Q01860

    - Isoform A: Q0...

    Read More

    Muchas gracias por tu respuesta.

    Es importante que todos los anticuerpos que escojas para llevar a cabo IHC-P en tejido humano estén validados (y por tanto garantizados por Abcam) para funcionar en esta especie y aplicación. Est...

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    Thank you for contacting Abcam. Regarding ab74538, when stored properly at -20, the antibody is expected to be stable for up to 5 years from receipt. I hope this information is helpful. Please do not hesitate to contact us if you have any additional qu...

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    Thank you for contacting Abcam regarding ab109183. I am sorry to hear that you have been experiencing difficulties with this antibody. Can you please provide some additional details as to the nature of the problems you are experiencing and your protoco...

    Read More

    Thank you for contacting Abcam about this. I have changed the datasheet to remedy the error that you have noticed. Ab109183 Anti-Oct4 antibody [EPR2054] is a rabbit IgG isotype. When using in Western Blot I would recommend using one of the followin...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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