Anti-Oct4 antibody [NRG1.1], prediluted (ab73099)

Overview

  • Product name
    Anti-Oct4 antibody [NRG1.1], prediluted
    See all Oct4 primary antibodies
  • Description
    Mouse monoclonal [NRG1.1] to Oct4, prediluted
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Oct4.

  • Positive control
    • Human seminoma tissue.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab73099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: Use neat for 30 minutes at room temperature.
    Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
    • Tissue specificity
      Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
    • Sequence similarities
      Belongs to the POU transcription factor family. Class-5 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Developmental stage
      Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
    • Domain
      The POU-specific domain mediates interaction with PKM2.
    • Post-translational
      modifications
      Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
      Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
    • Cellular localization
      Nucleus. Expressed in a diffuse and slightly punctuate pattern.
    • Information by UniProt
    • Database links
    • Alternative names
      • Octamer binding transcription factor 4 antibody
      • MGC22487 antibody
      • Oct 3 antibody
      • Oct 4 antibody
      • Oct-3 antibody
      • Oct-4 antibody
      • OCT3 antibody
      • Oct4 antibody
      • Octamer binding protein 3 antibody
      • Octamer binding protein 4 antibody
      • Octamer binding transcription factor 3 antibody
      • Octamer-binding protein 3 antibody
      • Octamer-binding protein 4 antibody
      • Octamer-binding transcription factor 3 antibody
      • OTF 3 antibody
      • OTF 4 antibody
      • OTF-3 antibody
      • OTF3 antibody
      • OTF4 antibody
      • PO5F1_HUMAN antibody
      • POU class 5 homeobox 1 antibody
      • POU domain class 5 transcription factor 1 antibody
      • POU domain transcription factor OCT4 antibody
      • POU domain, class 5, transcription factor 1 antibody
      • POU-type homeodomain-containing DNA-binding protein antibody
      • POU5F1 antibody
      see all

    Images

    • ab73099 staining Oct4 in human seminomatous tumor by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    References

    ab73099 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for contacting us.

    We recommend using Human seminoma tissue as positive control with this product. We currently have Testis tumor (human): seminoma tissue slides available on our catalog (http://www.abcam.com/testis-tumor-human-s...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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