Anti-Oct4 antibody [NRG1.1], prediluted (ab73099)

Overview

  • Product nameAnti-Oct4 antibody [NRG1.1], prediluted
    See all Oct4 primary antibodies
  • Description
    Mouse monoclonal [NRG1.1] to Oct4, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Oct4.

  • Positive control
    • Human seminoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab73099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: Use neat for 30 minutes at room temperature.
    Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
    • Tissue specificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
    • Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Developmental stageHighly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
    • DomainThe POU-specific domain mediates interaction with PKM2.
    • Post-translational
      modifications
      Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
      Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
    • Cellular localizationNucleus. Expressed in a diffuse and slightly punctuate pattern.
    • Information by UniProt
    • Database links
    • Alternative names
      • Octamer binding transcription factor 4 antibody
      • MGC22487 antibody
      • Oct 3 antibody
      • Oct 4 antibody
      • Oct-3 antibody
      • Oct-4 antibody
      • OCT3 antibody
      • Oct4 antibody
      • Octamer binding protein 3 antibody
      • Octamer binding protein 4 antibody
      • Octamer binding transcription factor 3 antibody
      • Octamer-binding protein 3 antibody
      • Octamer-binding protein 4 antibody
      • Octamer-binding transcription factor 3 antibody
      • OTF 3 antibody
      • OTF 4 antibody
      • OTF-3 antibody
      • OTF3 antibody
      • OTF4 antibody
      • PO5F1_HUMAN antibody
      • POU class 5 homeobox 1 antibody
      • POU domain class 5 transcription factor 1 antibody
      • POU domain transcription factor OCT4 antibody
      • POU domain, class 5, transcription factor 1 antibody
      • POU-type homeodomain-containing DNA-binding protein antibody
      • POU5F1 antibody
      see all

    Anti-Oct4 antibody [NRG1.1], prediluted images

    • ab73099 staining Oct4 in human seminomatous tumor by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    References for Anti-Oct4 antibody [NRG1.1], prediluted (ab73099)

    ab73099 has not yet been referenced specifically in any publications.

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    Thank you for contacting us.

    We recommend using Human seminoma tissue as positive control with this product. We currently have Testis tumor (human): seminoma tissue slides available on our catalog (http://www.abcam.com/testis-tumor-human-s...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"