This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 39 kDa.
1/200 - 1/1000.
FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Tissue specificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.
Developmental stageHighly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
DomainThe POU-specific domain mediates interaction with PKM2.
Post-translational modificationsSumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
Cellular localizationNucleus. Expressed in a diffuse and slightly punctuate pattern.
Indirect ELISA detection of immobilized Oct4 (full length) with titrated amounts of ab95854. No binding is seen to irrelevant recombinant proteins Nanog or SOX2.
Western blot - Anti-Oct4 [PR-2H9] antibody (ab95854)
Lane 1 : Anti-Oct4 antibody [PR-2H9] (ab95854) at 0.1 µg/ml Lanes 2 - 6 : Anti-Oct4 antibody [PR-2H9] (ab95854) at 1 µg/ml
Lane 1 : Recombinant Oct-4 protein at 0.25 µg Lane 2 : Recombinant Oct-4 protein at 0.25 µg Lane 3 : Untransfected HEK293 lysate at 20 µg Lane 4 : HEK293-Oct-4 transfectant cell lysate at 20 µg Lane 5 : NTERA-2 cell lysate at 20 µg Lane 6 : 2102 cell lysate at 20 µg
Predicted band size : 39 kDa
References for Anti-Oct4 antibody [PR-2H9] (ab95854)
has not yet been referenced specifically in any publications.
Publishing research using ab95854? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"