Overview

  • Product nameAnti-Oct4 antibody [PR-2H9]
    See all Oct4 primary antibodies
  • Description
    Mouse monoclonal [PR-2H9] to Oct4
  • Tested applicationsSuitable for: WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Oct4 aa 1-212.

  • Positive control
    • Recombinant Oct4 protein, HEK293-Oct-4 transfectant cell lysate, NTERA-2 cell lysate, and 2102 cell lysate.
  • General notes

    This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.

Properties

Applications

Our Abpromise guarantee covers the use of ab95854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 39 kDa.
Indirect ELISA 1/200 - 1/1000.

Target

  • FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Tissue specificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Developmental stageHighly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • DomainThe POU-specific domain mediates interaction with PKM2.
  • Post-translational
    modifications
    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • Cellular localizationNucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Information by UniProt
  • Database links
  • Alternative names
    • Octamer binding transcription factor 4 antibody
    • MGC22487 antibody
    • Oct 3 antibody
    • Oct 4 antibody
    • Oct-3 antibody
    • Oct-4 antibody
    • OCT3 antibody
    • Oct4 antibody
    • Octamer binding protein 3 antibody
    • Octamer binding protein 4 antibody
    • Octamer binding transcription factor 3 antibody
    • Octamer-binding protein 3 antibody
    • Octamer-binding protein 4 antibody
    • Octamer-binding transcription factor 3 antibody
    • OTF 3 antibody
    • OTF 4 antibody
    • OTF-3 antibody
    • OTF3 antibody
    • OTF4 antibody
    • PO5F1_HUMAN antibody
    • POU class 5 homeobox 1 antibody
    • POU domain class 5 transcription factor 1 antibody
    • POU domain transcription factor OCT4 antibody
    • POU domain, class 5, transcription factor 1 antibody
    • POU-type homeodomain-containing DNA-binding protein antibody
    • POU5F1 antibody
    see all

Anti-Oct4 antibody [PR-2H9] images

  • Indirect ELISA detection of immobilized Oct4 (full length) with titrated amounts of ab95854. No binding is seen to irrelevant recombinant proteins Nanog or SOX2.

  • Lane 1 : Anti-Oct4 antibody [PR-2H9] (ab95854) at 0.1 µg/ml
    Lanes 2 - 6 : Anti-Oct4 antibody [PR-2H9] (ab95854) at 1 µg/ml

    Lane 1 : Recombinant Oct-4 protein at 0.25 µg
    Lane 2 : Recombinant Oct-4 protein at 0.25 µg
    Lane 3 : Untransfected HEK293 lysate at 20 µg
    Lane 4 : HEK293-Oct-4 transfectant cell lysate at 20 µg
    Lane 5 : NTERA-2 cell lysate at 20 µg
    Lane 6 : 2102 cell lysate at 20 µg


    Predicted band size : 39 kDa

References for Anti-Oct4 antibody [PR-2H9] (ab95854)

ab95854 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"