Overview

  • Product nameAnti-Oct6 antibody
    See all Oct6 primary antibodies
  • Description
    Rabbit polyclonal to Oct6
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Oct6.

    (Peptide available as ab31795.)

  • Positive control
    • Mouse Brain, Mouse Brain Whole Cell Lysate - normal tissue, 0 days old, Rat Brain Whole Cell Lysate - normal tissue. This antibody gave a positive signal in the following Methanol fixed cell line: SKNSH.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab31766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
IHC-FoFr 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).

Target

  • FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis.
  • Tissue specificityExpressed in embryonal stem cells and in the developing brain.
  • Sequence similaritiesBelongs to the POU transcription factor family. Class-3 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • class 3 antibody
    • Oct-6 antibody
    • OCT6 antibody
    • Octamer-binding protein 6 antibody
    • Octamer-binding transcription factor 6 antibody
    • OTF-6 antibody
    • OTF6 antibody
    • PO3F1_HUMAN antibody
    • POU class 3 homeobox 1 antibody
    • POU domain antibody
    • POU domain class 3 transcription factor 1 antibody
    • POU domain transcription factor SCIP antibody
    • pou3f1 antibody
    • SCIP antibody
    • transcription factor 1 antibody
    see all

Anti-Oct6 antibody images

  • Lane 1 : Marker
    Lanes 2 - 4 : Anti-Oct6 antibody (ab31766) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : Mouse Brain at 20 µg
    Lane 3 : Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg
    Lane 4 : Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg

    Secondary
    Lanes 2 - 4 : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution


    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab31766 staining Oct6 in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.

    This image is courtesy of an Abreview submitted by Dr Sophie Pezet.

    See Abreview

  • ICC/IF image of ab31766 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31766 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Oct6 antibody (ab31766)

This product has been referenced in:
  • Inoue F  et al. Gbx2 directly restricts Otx2 expression to forebrain and midbrain, competing with class III POU factors. Mol Cell Biol 32:2618-27 (2012). Read more (PubMed: 22566684) »
  • Yasuhara N  et al. Triggering neural differentiation of ES cells by subtype switching of importin-alpha. Nat Cell Biol 9:72-9 (2007). ICC/IF ; Human . Read more (PubMed: 17159997) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (C57B/J mouse Vagus Nerve)
Specification C57B/J mouse Vagus Nerve
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton-X100 in PBS
Blocking step 5% BSA + 0.5% goat serum in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Kirk McManus

Verified customer

Submitted Mar 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jun 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"