Mouse Brain Whole Cell Lysate - normal tissue, 0 days old,
Rat Brain Whole Cell Lysate - normal tissue.
This antibody gave a positive signal in the following Methanol fixed cell line: SKNSH.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
ab31766 staining Oct6 in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.
This image is courtesy of an Abreview submitted by Dr Sophie Pezet.
ICC/IF image of ab31766 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31766 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Oct6 antibody (ab31766)
This product has been referenced in:
Inoue F et al. Gbx2 directly restricts Otx2 expression to forebrain and midbrain, competing with class III POU factors. Mol Cell Biol32:2618-27 (2012).
Read more (PubMed: 22566684) »
Yasuhara N et al. Triggering neural differentiation of ES cells by subtype switching of importin-alpha. Nat Cell Biol9:72-9 (2007).
Read more (PubMed: 17159997) »