Anti-Ocular development associated gene antibody [GATA9A1D3] (ab50816)
Key features and details
- Mouse monoclonal [GATA9A1D3] to Ocular development associated gene
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Ocular development associated gene antibody [GATA9A1D3] -
Description
Mouse monoclonal [GATA9A1D3] to Ocular development associated gene -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment (Human)
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Positive control
- HeLa and NIH3T3 cells or whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
GATA9A1D3 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/10.
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WB |
1/100. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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Notes |
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ICC/IF
1/10. |
WB
1/100. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Target
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Relevance
ODAG (Ocular development-associated gene), a novel transcription factor located on chromosome 7, encodes a protein that may play a role in eye development. mRNA profiling in multiple human tissue indicates that ODAG is expressed in human CD56+ NK cells and thyroid tissue. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 57798 Human
- Entrez Gene: 67210 Mouse
- SwissProt: Q8N5Y5 Human
- SwissProt: Q8WUU5 Human
- SwissProt: Q3TS14 Mouse
- SwissProt: Q8VCQ2 Mouse
- SwissProt: Q920S3 Mouse
- SwissProt: Q9CSG2 Mouse
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Alternative names
- 2310031E19Rik antibody
- 2810047M21Rik antibody
- 8430439A17Rik antibody
see all
Images
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Anti-Ocular development associated gene antibody [GATA9A1D3] (ab50816) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 29 kDa -
ab50816 at 1/10 dilution staining human GATAD1 in HeLa cells by Immunocytochemistry. AlexaFluor®488 Goat Anti-mouse IgG at 1/200 as secondary antibody.
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Anti-Ocular development associated gene antibody [GATA9A1D3] (ab50816) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab50816 has been referenced in 1 publication.
- Theis JL et al. Homozygosity mapping and exome sequencing reveal GATAD1 mutation in autosomal recessive dilated cardiomyopathy. Circ Cardiovasc Genet 4:585-94 (2011). ICC/IF ; Human . PubMed: 21965549