Anti-Ocular development associated gene antibody [GATA9A1D3] (ab50816)

Overview

  • Product nameAnti-Ocular development associated gene antibody [GATA9A1D3]
    See all Ocular development associated gene primary antibodies
  • Description
    Mouse monoclonal [GATA9A1D3] to Ocular development associated gene
  • Tested applicationsSuitable for: ICC/IF, Dot blot, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • HeLa and NIH3T3 cells or whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab50816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10.
Dot blot Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/100. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Target

  • RelevanceODAG (Ocular development-associated gene), a novel transcription factor located on chromosome 7, encodes a protein that may play a role in eye development. mRNA profiling in multiple human tissue indicates that ODAG is expressed in human CD56+ NK cells and thyroid tissue.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • 2310031E19Rik antibody
    • 2810047M21Rik antibody
    • 8430439A17Rik antibody
    • 9130430G15Rik antibody
    • B330017N08Rik antibody
    • ENSMUSG00000073241 antibody
    • FLJ22489 antibody
    • FLJ40695 antibody
    • GATA zinc finger domain containing 1 antibody
    • GATAD1 protein antibody
    • ocular development associated antibody
    • ODAG antibody
    • RG083M05.2 antibody
    • tcag7.279 antibody
    see all

Anti-Ocular development associated gene antibody [GATA9A1D3] images

  • Anti-Ocular development associated gene antibody [GATA9A1D3] (ab50816) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • ab50816 at 1/10 dilution staining human GATAD1 in HeLa cells by Immunocytochemistry. AlexaFluor®488 Goat Anti-mouse IgG at 1/200 as secondary antibody.
  • Anti-Ocular development associated gene antibody [GATA9A1D3] (ab50816) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 29 kDa
    Observed band size : 29 kDa

References for Anti-Ocular development associated gene antibody [GATA9A1D3] (ab50816)

This product has been referenced in:
  • Theis JL  et al. Homozygosity mapping and exome sequencing reveal GATAD1 mutation in autosomal recessive dilated cardiomyopathy. Circ Cardiovasc Genet 4:585-94 (2011). ICC/IF ; Human . Read more (PubMed: 21965549) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Zebrafish Embryo (heart)
Specification heart
Username

Abcam user community

Verified customer

Submitted Feb 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"