Overview

  • Product nameAnti-OGDH antibody
    See all OGDH primary antibodies
  • Description
    Rabbit polyclonal to OGDH
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N-terminal amino acids 2 - 51 (FHLRTCAAKL RPLTASQTVK TFSQNRPAAA RTFQQIRCYS APVAAEPFLS) of Human OGDH (NP_001003941)

  • Positive control
    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionThe 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Sequence similaritiesBelongs to the alpha-ketoglutarate dehydrogenase family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 oxoglutarate dehydrogenase antibody
    • 2 oxoglutarate dehydrogenase complex component E1 antibody
    • 2 oxoglutarate dehydrogenase mitochondrial antibody
    • 2-oxoglutarate dehydrogenase antibody
    • 2-oxoglutarate dehydrogenase complex component E1 antibody
    • AKGDH antibody
    • Alpha ketoglutarate dehydrogenase antibody
    • Alpha-ketoglutarate dehydrogenase antibody
    • E1k antibody
    • mitochondrial antibody
    • ODO1_HUMAN antibody
    • OGDC antibody
    • OGDC E1 antibody
    • OGDC-E1 antibody
    • OGDH antibody
    • Oxoglutarate (alpha ketoglutarate) dehydrogenase (lipoamide) antibody
    • Oxoglutarate decarboxylase antibody
    • Oxoglutarate dehydrogenase (lipoamide) antibody
    • Oxoglutarate dehydrogenase (succinyl transferring) antibody
    see all

Anti-OGDH antibody images

  • Anti-OGDH antibody (ab87057) at 1 µg/ml + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa

References for Anti-OGDH antibody (ab87057)

ab87057 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Prostate cancer cell line)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 25 µg
Specification Prostate cancer cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
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Submitted Jul 04 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"