Anti-OGDH antibody - C-terminal (ab137773)
Key features and details
- Rabbit polyclonal to OGDH - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-OGDH antibody - C-terminal
See all OGDH primary antibodies -
Description
Rabbit polyclonal to OGDH - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow -
Immunogen
Recombinant fragment, corresponding to a region within C terminal amino acids 705-1000 of Human OGDH (Uniprot ID: Q02218).
-
Positive control
- 293T, A431, H1299, HeLa S3, HepG2, MOLT4, Mouse brain and Raji whole cell lysates; HeLa cells; Human Cal27 xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 116 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). -
Sequence similarities
Belongs to the alpha-ketoglutarate dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 534599 Cow
- Entrez Gene: 4967 Human
- Entrez Gene: 18293 Mouse
- Entrez Gene: 360975 Rat
- Omim: 613022 Human
- SwissProt: Q148N0 Cow
- SwissProt: Q02218 Human
- SwissProt: Q60597 Mouse
see all -
Alternative names
- 2 oxoglutarate dehydrogenase antibody
- 2 oxoglutarate dehydrogenase complex component E1 antibody
- 2 oxoglutarate dehydrogenase mitochondrial antibody
see all
Images
-
All lanes : Anti-OGDH antibody - C-terminal (ab137773) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 116 kDa
7.5% SDS PAGE -
Anti-OGDH antibody - C-terminal (ab137773) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 116 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells labelling ODGH using ab137773 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
-
Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue labelling OGDH with ab135660 at 1/500 dilution
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (12)
ab137773 has been referenced in 12 publications.
- Pan Y et al. Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post-conditioning. Mol Med Rep 23:N/A (2021). PubMed: 33355377
- Yap ZY et al. A biallelic pathogenic variant in the OGDH gene results in a neurological disorder with features of a mitochondrial disease. J Inherit Metab Dis 44:388-400 (2021). PubMed: 32383294
- Hao Y et al. OGDH is involved in sepsis induced acute lung injury through the MAPK pathway. J Thorac Dis 13:5042-5054 (2021). PubMed: 34527342
- Yap ZY et al. Bi-allelic variants in OGDHL cause a neurodevelopmental spectrum disease featuring epilepsy, hearing loss, visual impairment, and ataxia. Am J Hum Genet 108:2368-2384 (2021). PubMed: 34800363
- Yang Q et al. Metabolic changes during malignant transformation in primary cells of oral lichen planus: Succinate accumulation and tumour suppression. J Cell Mol Med 24:1179-1188 (2020). PubMed: 31793175