Overview

  • Product nameAnti-Ogg1 antibody
    See all Ogg1 primary antibodies
  • Description
    Goat polyclonal to Ogg1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    C-HPKTSQAKGPSPLANK

    , corresponding to internal sequence amino acids 282-297 of Mouse Ogg1

  • Positive control
    • Human Kidney tissue Rat Spleen lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 39 kDa.
ELISA 1/128000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionDNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in OGG1 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
  • Sequence similaritiesBelongs to the type-1 OGG1 family.
  • Cellular localizationMitochondrion; Nucleus and Nucleus > nucleoplasm. Nucleus speckle. Nucleus matrix. Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 8 hydroxyguanine DNA glycosylase antibody
    • 8 oxoguanine DNA glycosylase 1 antibody
    • 8 oxoguanine DNA glycosylase antibody
    • AP lyase antibody
    • DNA (apurinic or apyrimidinic site) lyase antibody
    • DNA apurinic or apyrimidinic site lyase antibody
    • DNA lyase antibody
    • DNA-(apurinic or apyrimidinic site) lyase antibody
    • HMMH antibody
    • HOGG 1 antibody
    • HOGG1 antibody
    • MMH antibody
    • MUTM antibody
    • N glycosylase antibody
    • Ogg 1 antibody
    • Ogg1 antibody
    • OGG1_HUMAN antibody
    • OGH 1 antibody
    • OGH1 antibody
    see all

Anti-Ogg1 antibody images

  • Human Kidney (formalin-fixed, paraffin-embedded) stained with ab115841 at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-Ogg1 antibody (ab115841) at 0.1 µg/ml + Rat Spleen lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 39 kDa

References for Anti-Ogg1 antibody (ab115841)

ab115841 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Joy Mohanty

Verified customer

Submitted Oct 06 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"