Anti-Ogg1 antibody (ab62826)

Overview

  • Product name
  • Description
    Goat polyclonal to Ogg1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Cow, Human
  • Immunogen

    Synthetic peptide:

    C-HPKTSQAKGPSPLANK

    , corresponding to internal sequence amino acids 282-297 of Rat Ogg1

  • Positive control
    • Rat Spleen lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa).
ELISA 1/128000.

Target

  • Function
    DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in OGG1 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
  • Sequence similarities
    Belongs to the type-1 OGG1 family.
  • Cellular localization
    Mitochondrion; Nucleus and Nucleus > nucleoplasm. Nucleus speckle. Nucleus matrix. Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 8 hydroxyguanine DNA glycosylase antibody
    • 8 oxoguanine DNA glycosylase 1 antibody
    • 8 oxoguanine DNA glycosylase antibody
    • AP lyase antibody
    • DNA (apurinic or apyrimidinic site) lyase antibody
    • DNA apurinic or apyrimidinic site lyase antibody
    • DNA lyase antibody
    • DNA-(apurinic or apyrimidinic site) lyase antibody
    • HMMH antibody
    • HOGG 1 antibody
    • HOGG1 antibody
    • MMH antibody
    • MUTM antibody
    • N glycosylase antibody
    • Ogg 1 antibody
    • Ogg1 antibody
    • OGG1_HUMAN antibody
    • OGH 1 antibody
    • OGH1 antibody
    see all

Images

References

ab62826 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Cell lysate - nuclear (rat aortic smooth muscle cells)
Specification
rat aortic smooth muscle cells
Treatment
transfected
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jun 24 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - nuclear (mouse embryonic fibroblast cells)
Loading amount
35 µg
Specification
mouse embryonic fibroblast cells
Treatment
0, 1, 10, 100, 1000 uM BSO for 24 hours
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Dec 10 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - nuclear (MH1C1 cell (Rat hepatoma))
Loading amount
70 µg
Specification
MH1C1 cell (Rat hepatoma)
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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