Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Ogg1 aa 300 to the C-terminus (C terminal).
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab124741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Ogg1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124741 observed at 38 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab124741 was shown to recognize Ogg1 when Ogg1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ogg1 knockout samples were subjected to SDS-PAGE. ab124741 and ab18058 (loading control to vinculin) were diluted 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Blocking and dilution buffer: 5% NFDM/TBST.