Validated using a knockout cell line

Anti-Ogg1 antibody [EPR4664(2)] (ab124741)


  • Product name
    Anti-Ogg1 antibody [EPR4664(2)]
    See all Ogg1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4664(2)] to Ogg1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Ogg1 aa 300 to the C-terminus (C terminal).

  • Positive control
    • WB: HeLa, JAR, Raji, NCCIT and HepG2 cell lysates. Human cerebellum tissue lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab124741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
    • Tissue specificity
    • Involvement in disease
      Defects in OGG1 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
    • Sequence similarities
      Belongs to the type-1 OGG1 family.
    • Cellular localization
      Mitochondrion; Nucleus and Nucleus > nucleoplasm. Nucleus speckle. Nucleus matrix. Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • 8 hydroxyguanine DNA glycosylase antibody
      • 8 oxoguanine DNA glycosylase 1 antibody
      • 8 oxoguanine DNA glycosylase antibody
      • AP lyase antibody
      • DNA (apurinic or apyrimidinic site) lyase antibody
      • DNA apurinic or apyrimidinic site lyase antibody
      • DNA lyase antibody
      • DNA-(apurinic or apyrimidinic site) lyase antibody
      • HMMH antibody
      • HOGG 1 antibody
      • HOGG1 antibody
      • MMH antibody
      • MUTM antibody
      • N glycosylase antibody
      • Ogg 1 antibody
      • Ogg1 antibody
      • OGG1_HUMAN antibody
      • OGH 1 antibody
      • OGH1 antibody
      see all


    • Predicted band size : 39 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Ogg1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab124741 observed at 38 kDa. Red - loading control, ab18058, observed at 124 kDa.
      ab124741 was shown to recognize Ogg1 when Ogg1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ogg1 knockout samples were subjected to SDS-PAGE. ab124741 and ab18058 (loading control to vinculin) were diluted 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Ogg1 antibody [EPR4664(2)] (ab124741) at 1/50000 dilution (purified)

      Lane 1 : JAR cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : NCCIT cell lysate

      Lysates/proteins at 20 µg per lane.

      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size : 39 kDa
      Observed band size : 39 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Ogg1 antibody [EPR4664(2)] (ab124741) at 1/10000 dilution (unpurified)

      Lane 1 : Human cerebellum tissue lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 39 kDa
      Observed band size : 39 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Yang C  et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic Acids Res 45:739-748 (2017). Read more (PubMed: 27794043) »
    • Sassa A  et al. Xeroderma Pigmentosum Group A Suppresses Mutagenesis Caused by Clustered Oxidative DNA Adducts in the Human Genome. PLoS One 10:e0142218 (2015). WB ; Human . Read more (PubMed: 26559182) »

    See all 8 Publications for this product

    Customer reviews and Q&As

    Western blot
    Human Cell lysate - whole cell (Vascular Smooth Mucle Cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Vascular Smooth Mucle Cell
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted May 16 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Cell lysate - whole cell (various tumor cells types)
    Loading amount
    200000 cells
    various tumor cells types
    Gel Running Conditions
    Reduced Denaturing (10-20% Tris Glycine)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted May 02 2013

    The exact sequence of the immunogen used to raise this antibody (ab124741) is commercially sensitive information and unfortunately, I am unable to release it.
    However, I can confirm that the sequence corresponds to the isoform 1A of human OGG1.


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