Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab106319)

Overview

  • Product name
    Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
    See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies
  • Description
    Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human OGT/ O-Linked N-Acetylglucosamine Transferase.

  • Positive control
    • This antibody gave a positive signal in both HeLa and A549 whole cell lysates and HepG2 cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
  • Tissue specificity
    Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the O-GlcNAc transferase family.
    Contains 13 TPR repeats.
  • Domain
    The TPR repeat domain mediates recognition of protein substrates.
  • Post-translational
    modifications
    Ubiquitinated, leading to its proteasomal degradation.
  • Cellular localization
    Cytoplasm. Nucleus. Mostly in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ23071 antibody
    • GlcNAc transferase antibody
    • HRNT1 antibody
    • MGC22921 antibody
    • O GlcNAc antibody
    • O GlcNAc transferase p110 subunit antibody
    • O GlcNAc transferase subunit p110 antibody
    • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
    • O linked N acetylglucosamine (GlcNAc) transferase antibody
    • O linked N acetylglucosamine transferase 110 kDa subunit antibody
    • O-GlcNAc transferase subunit p110 antibody
    • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
    • ogt antibody
    • OGT1_HUMAN antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
    • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
    • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
    see all

Images

  • All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab106319) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 116 kDa
    Observed band size : 116 kDa
    Additional bands at : 170 kDa,72 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab106319 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106319, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) MCF7 cells at 5µg/ml.

References

ab106319 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab106319.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up