The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa).
1/50 - 1/100. (Cells fixed with paraformaldehyde/triton).
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
Protein modification; protein glycosylation.
Belongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
The TPR repeat domain mediates recognition of protein substrates.
Ubiquitinated, leading to its proteasomal degradation.