Purification notesWhole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa).
1/50 - 1/100. (Cells fixed with paraformaldehyde/triton).
FunctionAddition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Tissue specificityHighly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
DomainThe TPR repeat domain mediates recognition of protein substrates.
Post-translational modificationsUbiquitinated, leading to its proteasomal degradation.
Cellular localizationCytoplasm. Nucleus. Mostly in the nucleus.
Cytoplasmic, and nuclear localization of O-Linked N-Acetylglucosamine in human lung carcinoma (A549) cells stained by ab50270.
A. Working dilution of 1:100
B. Working dilution of 1:50
References for Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab50270)
This product has been referenced in:
Runager K et al. Targeting O-glycosyltransferase (OGT) to promote healing of diabetic skin wounds. J Biol Chem289:5462-6 (2014).
Read more (PubMed: 24398691) »
Rogacka D et al. Expression of GFAT1 and OGT in podocytes: transport of glucosamine and the implications for glucose uptake into these cells. J Cell Physiol225:577-84 (2010).
Read more (PubMed: 20506529) »