Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab50271)


  • Product nameAnti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
    See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies
  • Description
    Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:


    conjugated to KLH via an N-terminal added lysine residue, corresponding to amino acids 833-849 of Human O-Linked N-Acetylglucosamine

  • Positive control
    • HeLa cell nuclear extract. HeLa cell lysate. HEK 293T cell lysate.



Our Abpromise guarantee covers the use of ab50271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa). A 78kDa band may be seen in some preparations.
IP Use a concentration of 3 - 6 µg/ml.


  • FunctionAddition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
  • Tissue specificityHighly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
  • PathwayProtein modification; protein glycosylation.
  • Sequence similaritiesBelongs to the O-GlcNAc transferase family.
    Contains 13 TPR repeats.
  • DomainThe TPR repeat domain mediates recognition of protein substrates.
  • Post-translational
    Ubiquitinated, leading to its proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus. Mostly in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ23071 antibody
    • GlcNAc transferase antibody
    • HRNT1 antibody
    • MGC22921 antibody
    • O GlcNAc antibody
    • O GlcNAc transferase p110 subunit antibody
    • O GlcNAc transferase subunit p110 antibody
    • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
    • O linked N acetylglucosamine (GlcNAc) transferase antibody
    • O linked N acetylglucosamine transferase 110 kDa subunit antibody
    • O-GlcNAc transferase subunit p110 antibody
    • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
    • ogt antibody
    • OGT1_HUMAN antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
    • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
    • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
    see all

References for Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab50271)

This product has been referenced in:
  • Cox EJ & Marsh SA Exercise and diabetes have opposite effects on the assembly and O-GlcNAc modification of the mSin3A/HDAC1/2 complex in the heart. Cardiovasc Diabetol 12:101 (2013). Read more (PubMed: 23835259) »
  • Kebede M  et al. Glucose activates free fatty acid receptor 1 gene transcription via phosphatidylinositol-3-kinase-dependent O-GlcNAcylation of pancreas-duodenum homeobox-1. Proc Natl Acad Sci U S A 109:2376-81 (2012). WB ; Human, Mouse . Read more (PubMed: 22308370) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic stem cell)
Loading amount 20 µg
Specification Mouse embryonic stem cell
Gel Running Conditions Reduced Denaturing (4-12%, MOPS buffer)
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 20°C

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Submitted May 05 2012