The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at a concentration of 2.5 - 5 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa).
Suitable for Array.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAddition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Tissue specificityHighly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
DomainThe TPR repeat domain mediates recognition of protein substrates.
Post-translational modificationsUbiquitinated, leading to its proteasomal degradation.
Cellular localizationCytoplasm. Nucleus. Mostly in the nucleus.