Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab50273)

Overview

  • Product nameAnti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
    See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies
  • Description
    Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    DLKAFLDSLPDVKIVK

    conjugated to KLH by an N-terminal Cysteine residue linker, corresponding to amino acids 740-755 of Human O-Linked N-Acetylglucosamine

  • Positive control
    • HeLa cell nuclear extract HEK 293-T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at a concentration of 2.5 - 5 µg/ml.
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa).
    Suitable for Array.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAddition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
    • Tissue specificityHighly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
    • PathwayProtein modification; protein glycosylation.
    • Sequence similaritiesBelongs to the O-GlcNAc transferase family.
      Contains 13 TPR repeats.
    • DomainThe TPR repeat domain mediates recognition of protein substrates.
    • Post-translational
      modifications
      Ubiquitinated, leading to its proteasomal degradation.
    • Cellular localizationCytoplasm. Nucleus. Mostly in the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ23071 antibody
      • GlcNAc transferase antibody
      • HRNT1 antibody
      • MGC22921 antibody
      • O GlcNAc antibody
      • O GlcNAc transferase p110 subunit antibody
      • O GlcNAc transferase subunit p110 antibody
      • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
      • O linked N acetylglucosamine (GlcNAc) transferase antibody
      • O linked N acetylglucosamine transferase 110 kDa subunit antibody
      • O-GlcNAc transferase subunit p110 antibody
      • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
      • ogt antibody
      • OGT1_HUMAN antibody
      • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
      • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
      • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
      • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
      • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
      see all

    References for Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab50273)

    ab50273 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (293T, HepG2, MEF)
    Loading amount 20 µg
    Specification 293T, HepG2, MEF
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
    Username

    Mr. Dongil Kim

    Verified customer

    Submitted Feb 01 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"