The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAddition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Tissue specificityHighly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
DomainThe TPR repeat domain mediates recognition of protein substrates.
Post-translational modificationsUbiquitinated, leading to its proteasomal degradation.
Cellular localizationCytoplasm. Nucleus. Mostly in the nucleus.
References for Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135)
This product has been referenced in:
Moura CS et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One9:e83437 (2014).
Read more (PubMed: 24465380) »
Andrés-Bergós J et al. The increase in o-linked N-acetylglucosamine protein modification stimulates chondrogenic differentiation both in vitro and in vivo. J Biol Chem287:33615-28 (2012).
Read more (PubMed: 22859309) »