The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
Protein modification; protein glycosylation.
Belongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
The TPR repeat domain mediates recognition of protein substrates.
Ubiquitinated, leading to its proteasomal degradation.
References for Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135)
This product has been referenced in:
Moura CS et al. Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise. Food Nutr Res61:1290740 (2017).
Read more (PubMed: 28326005) »
Moura CS et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One9:e83437 (2014).
Read more (PubMed: 24465380) »