Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135)

Overview

  • Product name
    Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
    See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies
  • Description
    Goat polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
  • Specificity
    ab59135 is expected to recognize both reported isoforms of O-Linked N-Acetylglucosamine
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-YEHPKDLKLSDGR

    by a Cysteine residue linker, corresponding to amino acids 543-555 of Human O-Linked N-Acetylglucosamine

  • Positive control
    • Rat or mouse pancreas cytoplasmic or nuclear lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab59135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Antibody detection limit dilution 1:64,000.
    WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
    • Tissue specificity
      Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
    • Pathway
      Protein modification; protein glycosylation.
    • Sequence similarities
      Belongs to the O-GlcNAc transferase family.
      Contains 13 TPR repeats.
    • Domain
      The TPR repeat domain mediates recognition of protein substrates.
    • Post-translational
      modifications
      Ubiquitinated, leading to its proteasomal degradation.
    • Cellular localization
      Cytoplasm. Nucleus. Mostly in the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ23071 antibody
      • GlcNAc transferase antibody
      • HRNT1 antibody
      • MGC22921 antibody
      • O GlcNAc antibody
      • O GlcNAc transferase p110 subunit antibody
      • O GlcNAc transferase subunit p110 antibody
      • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
      • O linked N acetylglucosamine (GlcNAc) transferase antibody
      • O linked N acetylglucosamine transferase 110 kDa subunit antibody
      • O-GlcNAc transferase subunit p110 antibody
      • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
      • ogt antibody
      • OGT1_HUMAN antibody
      • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
      • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
      • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
      • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
      • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
      see all

    Images

    • Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135) at 0.05 µg/ml + Rat pancreas lysate in RIPA buffer at 35 µg

      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:
    • Moura CS  et al. Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise. Food Nutr Res 61:1290740 (2017). WB ; Rat . Read more (PubMed: 28326005) »
    • Moura CS  et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014). WB ; Rat . Read more (PubMed: 24465380) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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