Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab97274)

Overview

  • Product nameAnti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
    See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies
  • Description
    Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Zebrafish
  • Immunogen

    Synthetic peptide: MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHCMQLWRQ EPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYK ERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSAL QYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCV FNAQGEIWLAIHHFEKAVTLDPNFLD AYINLGNVLKEARI, corresponding to amino acids 1 - 240 of Human O-Linked N-Acetylglucosamine Transferase (NP_858059).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates This antibody gave a positive result when used in the following methanol fixed cell lines: DU145.

Properties

Applications

Our Abpromise guarantee covers the use of ab97274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 117 kDa.

Target

  • FunctionAddition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
  • Tissue specificityHighly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
  • PathwayProtein modification; protein glycosylation.
  • Sequence similaritiesBelongs to the O-GlcNAc transferase family.
    Contains 13 TPR repeats.
  • DomainThe TPR repeat domain mediates recognition of protein substrates.
  • Post-translational
    modifications
    Ubiquitinated, leading to its proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus. Mostly in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ23071 antibody
    • GlcNAc transferase antibody
    • HRNT1 antibody
    • MGC22921 antibody
    • O GlcNAc antibody
    • O GlcNAc transferase p110 subunit antibody
    • O GlcNAc transferase subunit p110 antibody
    • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
    • O linked N acetylglucosamine (GlcNAc) transferase antibody
    • O linked N acetylglucosamine transferase 110 kDa subunit antibody
    • O-GlcNAc transferase subunit p110 antibody
    • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
    • ogt antibody
    • OGT1_HUMAN antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
    • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
    • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
    see all

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody images

  • ICC/IF image of ab97274 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97274 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab97274) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 117 kDa
    7.5% SDS PAGE

References for Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab97274)

ab97274 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (neuroblastoma)
Specification neuroblastoma
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Submitted Apr 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"