Overview

  • Product nameAnti-OIF antibody
    See all OIF primary antibodies
  • Description
    Rabbit polyclonal to OIF
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 253-284 of Human OIF (NP_148935.1 , NP_054776.1).

  • Positive control
    • 293 cell lysate transiently transfected with the OIF gene; CEM cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab110558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 34 kDa.

Target

  • FunctionInduces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.
  • Tissue specificityBone.
  • Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily.
    Contains 7 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Corneal keratan sulfate proteoglycan antibody
    • DKFZP586P2421 antibody
    • MIME_HUMAN antibody
    • Mimecan antibody
    • Mimecan proteoglycan antibody
    • OG antibody
    • OGN antibody
    • OIF antibody
    • Osteoglycin antibody
    • Osteoinductive factor antibody
    • SLRR3A antibody
    see all

Anti-OIF antibody images

  • All lanes : Anti-OIF antibody (ab110558) at 1/100 dilution

    Lane 1 : Nontransfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the OIF gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 34 kDa
  • Anti-OIF antibody (ab110558) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 34 kDa

References for Anti-OIF antibody (ab110558)

This product has been referenced in:
  • Van Aelst LN  et al. Osteoglycin prevents cardiac dilatation and dysfunction after myocardial infarction through infarct collagen strengthening. Circ Res 116:425-36 (2015). WB ; Mouse . Read more (PubMed: 25520363) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Tissue lysate - whole (Aorta)
Specification Aorta
Treatment Non Deglycosylated, Deglycosylated using PNGaseF
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 16 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Adipose tissue)
Loading amount 20 µg
Specification Adipose tissue
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Maria Insenser

Verified customer

Submitted Jan 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"