Overview

  • Product name
    Oil Red O (Lipid Stain) Kit
  • Product overview

    Oil Red O (Lipid Stain) kit is intended for use in the histological visualization of fat cells and neutral fat. This kit may be used ONLY on frozen tissue sections, fresh smears, or touch preps. 


    If you prefer to use your own Hematoxylin and Propylene Glycol, we recommend our Oil Red O Solution ab223796.


    If you prefer to prepare your own Oil Red O solution, we recommend our solid Oil Red O stain ab146295.

  • Notes

    Staining Interpretation

      

    Fat Cells   Red
    Neutral Fat     Red
    Nuclei Blue

     

    Control Tissue: Any frozen section containing fat.

  • Tested applications
    Suitable for: IHC-Frmore details

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab150678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Images

  • Ab150678 (Oil Red O stain) staining lipid deposits (red) in frozen normal human skin. The dermis is unstained, whereas the hypodermis is stained, demonstrating dye specificity. Hematoxylin (blue) is used as a counterstain.  

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • During adipocyte differentiation, cells accumulate lipid. Oil Red O staining kit (ab150678) was used to visualize lipid in 3T3-L1 cells (left) and adipocyte-like cells (right). As expected, lipid accumulates in 3T3-L1 cells after differentiation into adipocyte-like cells.

Protocols

References

This product has been referenced in:
  • Xue L  et al. Duration of simulated microgravity affects the differentiation of mesenchymal stem cells. Mol Med Rep 15:3011-3018 (2017). Read more (PubMed: 28339035) »
  • Chalyk NE  et al. Morphological Characteristics of Residual Skin Surface Components Collected from the Surface of Facial Skin in Women of Different Age. Ann Dermatol 29:454-461 (2017). Read more (PubMed: 28761294) »

See all 5 Publications for this product

Customer reviews and Q&As

This reference contains a protocol for staining cultured cells with Oil Red O.

Hope R. G., McLauchlan J. (2000) J. Gen. Virol. 81:1913–1925.

http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-8-1913#tab2
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I am sorry to confirm that we do not know if our Oil Red O solution will work for live adipocytes. We do not have a protocol or data for this and most of the protocols I have found require alcoholic solutions (ab150678 does not) and a fixatio...

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The frozen tissues need to be fixed. Fixing in the PFA is fine. However, when fixing the tissue, avoid using reagents that will dissolve the fats.

We would recommend putting about 200 to 300ul of the solution (depending on the size of the spec...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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