Anti-Olfactory Marker Protein antibody (ab87338)

Overview

  • Product nameAnti-Olfactory Marker Protein antibody
    See all Olfactory Marker Protein primary antibodies
  • Description
    Rabbit polyclonal to Olfactory Marker Protein
  • Tested applicationsSuitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Goat, Hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Olfactory Marker Protein.

  • Positive control
    • This antibody gave a positive signal in Mouse and Rat Olfactory Bulb Tissue Lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab87338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
IHC-FoFr 1/1000.

Target

  • RelevanceOlfactory marker protein (OMP) is an abundant, 19kDa, cytosolic protein that is almost exclusively expressed in mature, functioning, olfactory neurons but not in the neural precursor basal cells. The OMP gene structure and protein sequence are highly conserved between mouse, rat and human. Its tissue specific expression in the receptor cells together with the results of the mouse knockout studies show that OMP represents a novel modulatory component of the odor detection/signal transduction cascade.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Olfactory marker protein antibody
    • Olfactory neuronal specific protein antibody
    • OMP antibody

Anti-Olfactory Marker Protein antibody images

  • IHC-FoFr image of Olfactory Marker Protein staining on mouse olfactor bulb using ab87338. The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat.

    See Abreview

  • Anti-Olfactory Marker Protein antibody (ab87338) at 1 µg/ml + Rat Olfactory Bulb Lysate at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • Anti-Olfactory Marker Protein antibody (ab87338) at 1 µg/ml + Mouse Olfactory Bulb Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 18 kDa


    Exposure time : 16 minutes

References for Anti-Olfactory Marker Protein antibody (ab87338)

This product has been referenced in:
  • Chandler JD  et al. Low-dose oral cadmium increases airway reactivity and lung neuronal gene expression in mice. Physiol Rep 4:N/A (2016). WB ; Mouse . Read more (PubMed: 27401458) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (Olfactory epithelium)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako citrate target retrieval buffer
Permeabilization No
Specification Olfactory epithelium
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 19 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Olfactory Epithelium)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako citrate antigen retrieval buffer
Permeabilization No
Specification Olfactory Epithelium
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 16 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain - Olfactory Bulb)
Specification Brain - Olfactory Bulb
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Mar 20 2012

We use 5% BSA for blocking and diluting the primary antibody, which for some antibodies will give a stronger signal than 5% milk. So you may want to try that switch, if you are using milk to block and dilute. I also suggest simply using more antibody (...

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