Anti-OLFM4 antibody (ab105861)

Overview

  • Product name
  • Description
    Rabbit polyclonal to OLFM4
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee, Monkey
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 400-450 (ASTGNMVISK LNDTTLQVLN TWYTKQYKPS ASNAFMVCGV LYATRTMNTR T) of Human OLFM4, conjugated to KLH.

  • Positive control
    • Human liver lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 57 kDa.

Target

  • Function
    An antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion.
  • Tissue specificity
    Expressed during myeloid lineage development. Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Highly expressed in pancreatic cancer tissues and shows an elevated expression level during the early S phase of the cell cycle. Also expressed at high levels in stomach cancer and colon cancer tissues.
  • Sequence similarities
    Contains 1 olfactomedin-like domain.
  • Domain
    The olfactomedin-like domain is involved in the interaction with cadherin.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Secreted > extracellular space. According to PubMed:15059901, found in mitochondrion and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antiapoptotic protein GW112 antibody
    • bA209J19.1 antibody
    • G-CSF-stimulated clone 1 protein antibody
    • GC1 antibody
    • GW112 antibody
    • hGC 1 antibody
    • hGC-1 antibody
    • hOLfD antibody
    • KIAA4294 antibody
    • olfactomedin 4 antibody
    • Olfactomedin-4 antibody
    • OlfD antibody
    • OLFM4 antibody
    • OLFM4_HUMAN antibody
    • OLM4 antibody
    • UNQ362 antibody
    see all

Images

  • All lanes : Anti-OLFM4 antibody (ab105861) at 2 µg/ml

    Lane 1 : Human liver lysate with no immunizing peptide
    Lane 2 : Human liver lysate with immunizing peptide


    Predicted band size : 57 kDa
  • ab105861, at 5 µg/ml, staining OLFM4 in formalin fixed, paraffin embedded colon tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody followed by alkaline phosphatase-streptavidin and chromogen.

References

ab105861 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your email. I have sent the lysates ab29889 with order number 1007989. I hope this will help.

I have now received a reply from lab. They have confirmed observing a 57kDa using human, rat and mouse liver extracts and 57 kD/ 40 kD band in the stomach extracts. I am OK for sending positive control lysates. However I would be interested in getting ...

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Thank you for your email. I have forwarded the data to my colleague in lab and now I am waiting a reply. I will contact you again soon; normally they write back to us within 24 hours. I have also done further search which I would be happy in sharing wi...

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Thanks you very much for contacting us. My colleagues has just forwarded your details to me. I am sorry to hear that you are observing multiple bands again. I will certainly look into this case further and would also like to discuss this case with l...

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Thank you for contacting us.  Below is the IHC protocol for ab105861.  I hope this helps, please let me know if you have any additonal questions.   Tissue Preparation: Formalin fixation and embedding in paraffin wax Tissue Sectioning: Make 4-μm section...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (stomach, duodenum, jejunum, ileum, colon)
Specification
stomach, duodenum, jejunum, ileum, colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Vector Antigen Unmasking Solution
Permeabilization
Yes - Vector Antigen Unmasking Solution, Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Ms. Gail Butler

Verified customer

Submitted Aug 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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