Overview

  • Product nameAnti-OLFM4 antibody
    See all OLFM4 primary antibodies
  • Description
    Rabbit polyclonal to OLFM4
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human OLFM4.

    (Peptide available as ab98247.)

  • Positive control
    • In Western Blot, ab85046 gave a positive signal in Human liver tissue lysate. In IHC, this antibody gave a positive signal in a Formalin-Fixed Paraffin-Embedded Human Prostate tissue section. In IF, this antibody gave a positive signal in T84 cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).

Target

  • FunctionAn antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion.
  • Tissue specificityExpressed during myeloid lineage development. Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Highly expressed in pancreatic cancer tissues and shows an elevated expression level during the early S phase of the cell cycle. Also expressed at high levels in stomach cancer and colon cancer tissues.
  • Sequence similaritiesContains 1 olfactomedin-like domain.
  • DomainThe olfactomedin-like domain is involved in the interaction with cadherin.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationSecreted > extracellular space. According to PubMed:15059901, found in mitochondrion and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antiapoptotic protein GW112 antibody
    • bA209J19.1 antibody
    • G-CSF-stimulated clone 1 protein antibody
    • GC1 antibody
    • GW112 antibody
    • hGC 1 antibody
    • hGC-1 antibody
    • hOLfD antibody
    • KIAA4294 antibody
    • olfactomedin 4 antibody
    • Olfactomedin-4 antibody
    • OlfD antibody
    • OLFM4 antibody
    • OLFM4_HUMAN antibody
    • OLM4 antibody
    • UNQ362 antibody
    see all

Anti-OLFM4 antibody images

  • ab85046 stained T84 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85046 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-OLFM4 antibody (ab85046) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa


    Exposure time : 20 minutes
  • IHC image of OLFM4 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85046, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-OLFM4 antibody (ab85046)

This product has been referenced in:
  • Fields S  et al. New candidate biomarkers in the female genital tract to evaluate microbicide toxicity. PLoS One 9:e110980 (2014). Read more (PubMed: 25333937) »
  • Cheung EC  et al. TIGAR is required for efficient intestinal regeneration and tumorigenesis. Dev Cell 25:463-77 (2013). ICC ; Mouse . Read more (PubMed: 23726973) »

See all 4 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Vagina)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 40 µg
Specification Vagina
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Ms. Bareza Rasoul

Verified customer

Submitted Mar 28 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate or TRIS/EDTA buffer
Sample Mouse Tissue sections (small intestine)
Specification small intestine
Permeabilization No
Fixative Paraformaldehyde
Username

Ms. Pamela Riemer

Verified customer

Submitted Jan 14 2014

Thank you for your reply. I am very pleased to hear you would like to accept our offer and test ab85046 in mouse.

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Thank you for your message. I am sorry to hear that the availabilityissue for ab85046 Anti-OLFM4 antibodyis significantly affecting your work. I appreciate your concerns and apologise for the inconvenience.

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DISCOUNT CODE: XXXXXXXXX
Expiration date: 09-06-2012

I am very pleased to hear you would like to accept our offer and test ab85046 in Mouse. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"