• Product nameAnti-OLFM4 antibody
    See all OLFM4 primary antibodies
  • Description
    Rabbit polyclonal to OLFM4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 400-419 of Human OLFM4 (Gen Bank. gb|AAH47740.1).

  • Positive control
    • Human liver lysate or colon tissue


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab96280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 3 µg/ml. Predicted molecular weight: 57 kDa.

Load no more than 25ug of protein in WB.

IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • FunctionAn antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion.
  • Tissue specificityExpressed during myeloid lineage development. Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Highly expressed in pancreatic cancer tissues and shows an elevated expression level during the early S phase of the cell cycle. Also expressed at high levels in stomach cancer and colon cancer tissues.
  • Sequence similaritiesContains 1 olfactomedin-like domain.
  • DomainThe olfactomedin-like domain is involved in the interaction with cadherin.
  • Post-translational
  • Cellular localizationSecreted > extracellular space. According to PubMed:15059901, found in mitochondrion and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antiapoptotic protein GW112 antibody
    • bA209J19.1 antibody
    • G-CSF-stimulated clone 1 protein antibody
    • GC1 antibody
    • GW112 antibody
    • hGC 1 antibody
    • hGC-1 antibody
    • hOLfD antibody
    • KIAA4294 antibody
    • olfactomedin 4 antibody
    • Olfactomedin-4 antibody
    • OlfD antibody
    • OLFM4 antibody
    • OLFM4_HUMAN antibody
    • OLM4 antibody
    • UNQ362 antibody
    see all

Anti-OLFM4 antibody images

  • All lanes : Anti-OLFM4 antibody (ab96280) at 2 µg/ml

    Lane 1 : Human liver lysate
    Lane 2 : Human liver lysate with immunizing peptide

    Predicted band size : 57 kDa
  • Immunohistochemical analysis of OLFM4 in formalin-fixed, paraffin-embedded Human colon tissue using an isotype control (top) and ab96280 (bottom) at 5 ug/ml.

References for Anti-OLFM4 antibody (ab96280)

This product has been referenced in:
  • Amirbeagi F  et al. Olfactomedin-4 autoantibodies give unusual c-ANCA staining patterns with reactivity to a subpopulation of neutrophils. J Leukoc Biol 97:181-9 (2015). Read more (PubMed: 25387833) »
  • Giammanco A  et al. Intestinal epithelial HuR modulates distinct pathways of proliferation and apoptosis and attenuates small intestinal and colonic tumor development. Cancer Res 74:5322-35 (2014). Mouse . Read more (PubMed: 25085247) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Mouse Tissue lysate - whole (Spleen, liver, brian, vagina)
Specification Spleen, liver, brian, vagina
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Ms. Bareza Rasoul

Verified customer

Submitted Mar 27 2014

I have issued a free of charge replacement.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (intestine)
Specification intestine
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 16 2013

Code de remise : xxxxxxxxx
Date d’expiration : 29/03/13

Je suis ravie que vous souhaitiez participer a notre offre et tester ab96280 chez la souris. Le code de réduction correspondant est xxxxxxxx. Ce code de réducti...

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Merci de nous avoir contactés.

Je suis désolée qu’ab85046 soit en rupture de stock.

Je vous propose ab96280 (http://www.abcam.com/ab96280) qui a été testé en immunohistochemie sur coup...

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Thank you for your email. I have sent the lysates ab29889 with order number 1007989. I hope this will help.

I have now received a reply from lab. They have confirmed observing a 57kDa using human, rat and mouse liver extracts and 57 kD/ 40 kD band in the stomach extracts. I am OK for sending positive control lysates. However I would be interested in getting ...

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Thank you for your email. I have forwarded the data to my colleague in lab and now I am waiting a reply. I will contact you again soon; normally they write back to us within 24 hours. I have also done further search which I would be happy in sharing wi...

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Thanks you very much for contacting us. My colleagues has just forwarded your details to me. I am sorry to hear that you are observing multiple bands again. I will certainly look into this case further and would also like to discuss this case with l...

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Thank you for contacting us. We normally use 10% normal serum in 1%BSA and TBS for 1-2 hours RT. I would also recomend blcoking endogenous peroxidases if HRP detection system is being used.   I hope this information is helpful to you. Please do...

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