Overview

  • Product nameAnti-OLFML2A antibody
    See all OLFML2A primary antibodies
  • Description
    Rabbit polyclonal to OLFML2A
  • Specificityab75882 detects endogenous levels of total OLFML2A protein.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized peptide derived from internal of human OLFML2A.

  • Positive control
    • Extracts from RAW264.7 cells A549 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 73 kDa.
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceOLFML2A contains one olfactomedin-like domain. Its exact function is unknown. There are three named isoforms produced by alternative splicing.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • FLJ00237 antibody
    • Olfactomedin like 2A antibody
    • Olfactomedin like protein 2A antibody
    • OLM2A_HUMAN antibody
    • Photomedin 1 antibody
    • PRO34319 antibody
    • UNQ9394/PRO34319 antibody
    see all

Anti-OLFML2A antibody images

  • ab75882 staining OLFML2A in Human retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 12 hours at 25°C. Samples were incubated with primary antibody (1/500) for 2 hours at 25°C. A Cy3®-conjugated anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-OLFML2A antibody (ab75882) at 1/500 dilution

    Lane 1 : extracts from RAW264.7 cells
    Lane 2 : extracts from RAW264.7 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • ab75882, at a dilution of 1/500-1/1000, staining OLFML2A in A549 cells by Immunofluorescence with (+) or without (-) immunising peptide.

References for Anti-OLFML2A antibody (ab75882)

This product has been referenced in:
  • Pérez-Ibave DC  et al. Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) expression profile in primates (human and baboon). Biol Res 49:44 (2016). ICC/IF ; Human . Read more (PubMed: 27821182) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Retina)
Specification Retina
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
Username

Prof. IRAM PABLO RODRIGUEZ-SANCHEZ

Verified customer

Submitted Nov 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"