Overview

  • Product nameAnti-OLFML2A antibody
    See all OLFML2A primary antibodies
  • Description
    Rabbit polyclonal to OLFML2A
  • Specificityab75882 detects endogenous levels of total OLFML2A protein.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized peptide derived from internal of human OLFML2A.

  • Positive control
    • Extracts from RAW264.7 cells A549 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 73 kDa.
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceOLFML2A contains one olfactomedin-like domain. Its exact function is unknown. There are three named isoforms produced by alternative splicing.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • FLJ00237 antibody
    • Olfactomedin like 2A antibody
    • Olfactomedin like protein 2A antibody
    • OLM2A_HUMAN antibody
    • Photomedin 1 antibody
    • PRO34319 antibody
    • UNQ9394/PRO34319 antibody
    see all

Anti-OLFML2A antibody images

  • ab75882 staining OLFML2A in Human retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 12 hours at 25°C. Samples were incubated with primary antibody (1/500) for 2 hours at 25°C. A Cy3®-conjugated anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-OLFML2A antibody (ab75882) at 1/500 dilution

    Lane 1 : extracts from RAW264.7 cells
    Lane 2 : extracts from RAW264.7 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • ab75882, at a dilution of 1/500-1/1000, staining OLFML2A in A549 cells by Immunofluorescence with (+) or without (-) immunising peptide.

References for Anti-OLFML2A antibody (ab75882)

ab75882 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Retina)
Specification Retina
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
Username

Prof. IRAM PABLO RODRIGUEZ-SANCHEZ

Verified customer

Submitted Nov 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"