Overview

  • Product nameAnti-OLFML2B antibody
    See all OLFML2B primary antibodies
  • Description
    Rabbit polyclonal to OLFML2B
  • SpecificityDetects endogenous levels of total OLFML2B protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal region of human OLFML2B.

  • Positive control
    • Extracts from HUVEC cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
ELISA 1/10000.

Target

Anti-OLFML2B antibody images

  • All lanes : Anti-OLFML2B antibody (ab75355) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HUVEC cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Additional bands at : 48 kDa,65 kDa,86 kDa. We are unsure as to the identity of these extra bands.
  • ab75355 staining OLFML2B in Human retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with sodium borohydride and blocked with 5% milk for 2 hours at 25°C. Samples were incubated with primary antibody (undiluted) for 2 hours. A Cy3®-conjugated anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-OLFML2B antibody (ab75355)

ab75355 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Human Tissue sections (Retina)
Specification Retina
Permeabilization Yes - sodium borohydride
Fixative Paraformaldehyde
Username

Prof. IRAM PABLO RODRIGUEZ-SANCHEZ

Verified customer

Submitted Nov 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"