The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 23840470
1/100 - 1/600.
FunctionPromotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube.
Tissue specificityExpressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable.
ab68105, at 1/100 dilution, staining Olig1 in the white matter of adult rat spinal cord by Immunohistochemistry on cryo-preserved sections, using anti-rabbit Cy3-conjugated secondary antibodies (red) and DAPI (blue) as a nuclear counterstain.
References for Anti-Olig1 antibody (ab68105)
This product has been referenced in:
Szymanski CR et al. Paranode Abnormalities and Oxidative Stress in Optic Nerve Vulnerable to Secondary Degeneration: Modulation by 670 nm Light Treatment. PLoS One8:e66448 (2013).
Read more (PubMed: 23840470) »
Jakovcevski I & Zecevic N Olig transcription factors are expressed in oligodendrocyte and neuronal cells in human fetal CNS. J Neurosci25:10064-73 (2005).
Read more (PubMed: 16267213) »