Overview

  • Product nameAnti-Olig1 antibody [EPR6790]
    See all Olig1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6790] to Olig1
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • MOLT4, SHSY-5Y, 293T and C6 cell lysates; 293T cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionPromotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube.
    • Tissue specificityExpressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable.
    • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic domain helix loop helix protein class B 6 antibody
      • Basic domain helix loop helix protein class B6 antibody
      • BHLH B6 antibody
      • BHLHB 6 antibody
      • bHLHb6 antibody
      • bHLHe21 antibody
      • Class B basic helix-loop-helix protein 6 antibody
      • Class E basic helix-loop-helix protein 21 antibody
      • Olig 1 antibody
      • Olig1 antibody
      • OLIG1_HUMAN antibody
      • Oligo 1 antibody
      • Oligo1 antibody
      • Oligodendrocyte lineage transcription factor 1 antibody
      • Oligodendrocyte specific bHLH transcription factor 1 antibody
      • Oligodendrocyte transcription factor 1 antibody
      see all

    Anti-Olig1 antibody [EPR6790] images

    • All lanes : Anti-Olig1 antibody [EPR6790] (ab124908) at 1/1000 dilution

      Lane 1 : MOLT4 cell lysate
      Lane 2 : SHSY-5Y cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 27 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized 293T cells using ab124908 at 1/10 dilution (red) or a rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Olig1 antibody [EPR6790] (ab124908)

    This product has been referenced in:
    • Cheng T  et al. Effect of OLIG1 on the development of oligodendrocytes and myelination in a neonatal rat PVL model induced by hypoxia-ischemia. Mol Med Rep 11:2379-86 (2015). WB, IHC ; Rat . Read more (PubMed: 25435330) »

    See 1 Publication for this product

    Product Wall



    Not sure exactly why Olig1 runs a bit slower than would be suggested based on theoretical mass alone. It doesn't appear to be post translationally modified. But another Olig1 antibody in our catalog, ab97507 http://www.abcam.com/Olig1-antibod...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"