Anti-Olig1 antibody - N-terminal (ab191694)

Overview

  • Product name
    Anti-Olig1 antibody - N-terminal
    See all Olig1 primary antibodies
  • Description
    Rabbit polyclonal to Olig1 - N-terminal
  • Specificity
    ab191694 is predicted to not cross-react with other members of the OLIG family of proteins.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Olig1 (N terminal). The exact sequence is proprietary. Peptide corresponds to 18 amino acids near the amino terminus (NP_620450).
    Database link: Q8TAK6

  • Positive control
    • A549 cell lysate and Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab191694 is affinity chromatography purified via peptide column.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 28 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube.
  • Tissue specificity
    Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic domain helix loop helix protein class B 6 antibody
    • Basic domain helix loop helix protein class B6 antibody
    • BHLH B6 antibody
    • BHLHB 6 antibody
    • bHLHb6 antibody
    • bHLHe21 antibody
    • Class B basic helix-loop-helix protein 6 antibody
    • Class E basic helix-loop-helix protein 21 antibody
    • Olig 1 antibody
    • Olig1 antibody
    • OLIG1_HUMAN antibody
    • Oligo 1 antibody
    • Oligo1 antibody
    • Oligodendrocyte lineage transcription factor 1 antibody
    • Oligodendrocyte specific bHLH transcription factor 1 antibody
    • Oligodendrocyte transcription factor 1 antibody
    see all

Images

  • Anti-Olig1 antibody - N-terminal (ab191694) at 1 µg/ml + A549 cell lysate at 15 µg
    Developed using the ECL technique

    Predicted band size : 28 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of human brain tissue, labeling Olig1 using ab191694 at 20 μg/mL.

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue, labeling Olig1 using ab191694 at 5 μg/mL.

References

ab191694 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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