Recombinant
RabMAb

Anti-Olig2 antibody [EPR2673] (ab109186)

Overview

  • Product name
    Anti-Olig2 antibody [EPR2673]
    See all Olig2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2673] to Olig2
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in human Olig2.

  • Positive control
  • General notes

     

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 32 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
    • Tissue specificity
      Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
    • Involvement in disease
      Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
    • Sequence similarities
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Domain
      The bHLH is essential for interaction with NKX2-2.
    • Cellular localization
      Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic domain helix loop helix protein class B 1 antibody
      • Basic helix loop helix protein class B 1 antibody
      • BHLHB antibody
      • bHLHB1 antibody
      • bHLHe19 antibody
      • Class B basic helix loop helix protein 1 antibody
      • Class B basic helix-loop-helix protein 1 antibody
      • class E basic helix loop helix protein 19 antibody
      • Class E basic helix-loop-helix protein 19 antibody
      • Human protein kinase C binding protein RACK17 antibody
      • Olig2 antibody
      • OLIG2_HUMAN antibody
      • Oligo2 antibody
      • Oligodendrocyte lineage transcription factor 2 antibody
      • Oligodendrocyte specific bHLH transcription factor 2 antibody
      • Oligodendrocyte transcription factor 2 antibody
      • OTTHUMP00000067569 antibody
      • OTTHUMP00000067570 antibody
      • PRKCBP2 antibody
      • Protein kinase C binding protein 2 antibody
      • Protein kinase C binding protein RACK17 antibody
      • Protein kinase C-binding protein 2 antibody
      • Protein kinase C-binding protein RACK17 antibody
      • RACK17 antibody
      see all

    Anti-Olig2 antibody [EPR2673] images

    • All lanes : Anti-Olig2 antibody [EPR2673] (ab109186) at 1/2000 dilution (purified)

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 32 kDa
      Observed band size : 32 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-Olig2 antibody [EPR2673] (ab109186) at 1/10000 dilution (purified) + Human oligodendroglioma lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 32 kDa
      Observed band size : 32 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-Olig2 antibody [EPR2673] (ab109186) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 32 kDa
      Observed band size : 32 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Unpurified ab109186 staining Olig2 in rat brain tissue sections by immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in 0.1M PBS and blocked with 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/200 in PBS + 10% donkey serum) for 4 hours at 24°C. An Alexa Fluor® 488-conjugated anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei stained with Hoechst (blue).

      See Abreview

    • Anti-Olig2 antibody [EPR2673] (ab109186) at 1/1000 dilution (unpurified) + Oligodendroglioma lysate at 10 µg

      Performed under reducing conditions.

      Predicted band size : 32 kDa
    • Immunohistochemical staining of Olig2 in human glioma tissue with ab109186 at a dilution of 1/100.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Olig2 antibody [EPR2673] (ab109186)

    This product has been referenced in:
    • Yuan J  et al. M2 microglia promotes neurogenesis and oligodendrogenesis from neural stem/progenitor cells via the PPAR? signaling pathway. Oncotarget 8:19855-19865 (2017). WB, ICC/IF ; Mouse . Read more (PubMed: 28423639) »
    • Connolly NP  et al. Genetically engineered rat gliomas: PDGF-driven tumor initiation and progression in tv-a transgenic rats recreate key features of human brain cancer. PLoS One 12:e0174557 (2017). IHC-Fr ; Rat . Read more (PubMed: 28358926) »

    See all 6 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Rat Tissue sections (Brain Sections)
    Specification
    Brain Sections
    Permeabilization
    Yes - 0.1M PBS with 3% TritonX100
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Oct 02 2014

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain section)
    Specification
    Brain section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 14 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Spinal cord)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH6
    Permeabilization
    No
    Specification
    Spinal cord
    Blocking step
    Ultratek HRP (anti-polyvalent) kit, Scytek laboratories as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Username

    Sara Belluco

    Verified customer

    Submitted Apr 07 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1
    Permeabilization
    No
    Specification
    Brain
    Fixative
    formalin
    Username

    Dr. Emmanuel Bouchaert

    Verified customer

    Submitted Jan 03 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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