Overview

  • Product nameAnti-Olig3 antibody
    See all Olig3 primary antibodies
  • Description
    Rabbit polyclonal to Olig3
  • Tested applicationsSuitable for: IHC-Fr, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 53-66 of Human Olig3

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: 50% Ammonium sulphate, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesAntibody is affinity purified but presented as an ammonium sulfate precipitated purified material for stability.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
ICC
  • Application notesICC: 1/200 - 1/1000.
    IHC-Fr: 1/200 - 1/1000 for 90 minutes. Cut 10um slices on a cryostat and dry slides at RT for 30-90 minutes. Fix with cold Acetone/Methanol (50/50) for 2 minutes or alternatively with 4% PFA followed by treatment with a 0.05% trypsin solution (incubate for 10 minutes at 37°C, then wash 3 times with PBS)
    WB: 1/200 - 1/1000. Predicted molecular weight: 29 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
    • Sequence similaritiesContains 1 bHLH (basic helix-loop-helix) domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic helix loop helix domain containing class B protein 7 antibody
      • Basic helix-loop-helix domain-containing protein, class B, 7 antibody
      • bHLHB7 antibody
      • bHLHe20 antibody
      • Class B basic helix loop helix protein 7 antibody
      • Class B basic helix-loop-helix protein 7 antibody
      • Class E basic helix loop helix protein 20 antibody
      • Class E basic helix-loop-helix protein 20 antibody
      • Olig3 antibody
      • OLIG3_HUMAN antibody
      • Oligo3 antibody
      • Oligodendrocyte lineage transcription factor 3 antibody
      • Oligodendrocyte specific bHLH transcription factor 3 antibody
      • Oligodendrocyte transcription factor 3 antibody
      see all

    Anti-Olig3 antibody images

    • Confocal IF analysis of PC12 celss using ab78006 (Red). Actin filaments have been labeled with Alexa Fluor 488 - Phalloidin (Green). Nucleus is stained with DAPI (Blue).


    • Predicted band size : 29 kDa

    References for Anti-Olig3 antibody (ab78006)

    ab78006 has not yet been referenced specifically in any publications.

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    Zwar haben wir noch keine Publikation für diesen Antikörper in IHC, haben diesen Antikörper aber bisher erfolgreich in Kyrosektionen getestet, und können Ihnen daher fuer diese Applikation unsere Garantie in der...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"