Overview

  • Product name
  • Description
    Rabbit polyclonal to Olig3
  • Tested applications
    Suitable for: IHC-Fr, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 53-66 of Human Olig3

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituents: 50% Ammonium sulphate, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Antibody is affinity purified but presented as an ammonium sulfate precipitated purified material for stability.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
ICC
  • Application notes
    ICC: 1/200 - 1/1000.
    IHC-Fr: 1/200 - 1/1000 for 90 minutes. Cut 10um slices on a cryostat and dry slides at RT for 30-90 minutes. Fix with cold Acetone/Methanol (50/50) for 2 minutes or alternatively with 4% PFA followed by treatment with a 0.05% trypsin solution (incubate for 10 minutes at 37°C, then wash 3 times with PBS)
    WB: 1/200 - 1/1000. Predicted molecular weight: 29 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
    • Sequence similarities
      Contains 1 bHLH (basic helix-loop-helix) domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic helix loop helix domain containing class B protein 7 antibody
      • Basic helix-loop-helix domain-containing protein, class B, 7 antibody
      • bHLHB7 antibody
      • bHLHe20 antibody
      • Class B basic helix loop helix protein 7 antibody
      • Class B basic helix-loop-helix protein 7 antibody
      • Class E basic helix loop helix protein 20 antibody
      • Class E basic helix-loop-helix protein 20 antibody
      • Olig3 antibody
      • OLIG3_HUMAN antibody
      • Oligo3 antibody
      • Oligodendrocyte lineage transcription factor 3 antibody
      • Oligodendrocyte specific bHLH transcription factor 3 antibody
      • Oligodendrocyte transcription factor 3 antibody
      see all

    Images

    • Confocal IF analysis of PC12 celss using ab78006 (Red). Actin filaments have been labeled with Alexa Fluor 488 - Phalloidin (Green). Nucleus is stained with DAPI (Blue).


    • Predicted band size : 29 kDa

    References

    ab78006 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Vielen Dank für Ihre Anfrage.

    Zwar haben wir noch keine Publikation für diesen Antikörper in IHC, haben diesen Antikörper aber bisher erfolgreich in Kyrosektionen getestet, und können Ihnen daher fuer diese Applikation unsere Garantie in der...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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