Anti-Oligodendrocyte myelin glycoprotein antibody (ab224254)
Key features and details
- Rabbit polyclonal to Oligodendrocyte myelin glycoprotein
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Oligodendrocyte myelin glycoprotein antibody
See all Oligodendrocyte myelin glycoprotein primary antibodies -
Description
Rabbit polyclonal to Oligodendrocyte myelin glycoprotein -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human Oligodendrocyte myelin glycoprotein aa 1-200.
Database link: P23515 -
Positive control
- IHC: Human cerebral cortex and caudate nucleas tissue. ICC: U-251 MG sp cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system. -
Tissue specificity
Oligodendrocytes and myelin of the central nervous system. -
Sequence similarities
Contains 8 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
Post-translational
modificationsO-glycosylated in its Ser/Thr-rich repeat domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4974 Human
- Entrez Gene: 18377 Mouse
- Omim: 164345 Human
- SwissProt: P23515 Human
- SwissProt: Q63912 Mouse
- Unigene: 113874 Human
- Unigene: 93335 Mouse
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Alternative names
- Oligodendrocyte-myelin glycoprotein antibody
- Omg antibody
- OMGP antibody
- OMGP_HUMAN antibody
Images
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Paraffin-embedded human caudate nucleus stained for OMG using ab224254 at 1/1000 dilution in immunohistochemical analysis. Shows moderate to strong positivity in neuronal processes.
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Paraffin-embedded human kidney stained for OMG using ab224254 at 1/1000 dilution in immunohistochemical analysis. Shows no positivity in cells in tubules as expected.
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Paraffin-embedded human liver stained for OMG using ab224254 at 1/1000 dilution in immunohistochemical analysis. Shows no positivity in in hepatocytes as expected.
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Paraffin-embedded human cerebral cortex stained for OMG using ab224254 at 1/1000 dilution in immunohistochemical analysis. Shows moderate positivity in neurophil, as well as glial cells.
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All lanes : Anti-Oligodendrocyte myelin glycoprotein antibody (ab224254) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 50 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224254 has not yet been referenced specifically in any publications.